Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1007/s10295-015-1615-8
Volltext Shareable Link
Titel (primär) Making variability less variable: matching expression system and host for oxygenase-based biotransformations
Autor Lindmeyer, M.; Meyer, D.; Kuhn, D.; Bühler, B.; Schmid, A.
Quelle Journal of Industrial Microbiology & Biotechnology
Erscheinungsjahr 2015
Department SOMA
Band/Volume 42
Heft 6
Seite von 851
Seite bis 866
Sprache englisch
Supplements https://static-content.springer.com/esm/art%3A10.1007%2Fs10295-015-1615-8/MediaObjects/10295_2015_1615_MOESM1_ESM.pdf
Keywords Pseudomonas putida; Monooxygenase; Clonal variability; Solvent tolerance; alk-regulatory system
UFZ Querschnittsthemen RU4;
Abstract Variability in whole-cell biocatalyst performance represents a critical aspect for stable and productive bioprocessing. In order to investigate whether and how oxygenase-catalyzed reactions are affected by such variability issues in solvent-tolerant Pseudomonas, different inducers, expression systems, and host strains were tested for the reproducibility of xylene and styrene monooxygenase catalyzed hydroxylation and epoxidation reactions, respectively. Significantly higher activity variations were found for biocatalysts based on solvent-tolerant Pseudomonas putida DOT-TIE and S12 compared with solvent-sensitive P. putida KT2440, Escherichia coli JM101, and solvent-tolerant Pseudomonas taiwanensis VLB120. Specific styrene epoxidation rates corresponded to cellular styrene monooxygenase contents. Detected variations in activity strictly depended on the type of regulatory system employed, being high with the alk- and low with the lac-system. These results show that the occurrence of clonal variability in recombinant gene expression in Pseudomonas depends on the combination of regulatory system and host strain, does not correlate with a general phenotype such as solvent tolerance, and must be evaluated case by case.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16215
Lindmeyer, M., Meyer, D., Kuhn, D., Bühler, B., Schmid, A. (2015):
Making variability less variable: matching expression system and host for oxygenase-based biotransformations
J. Ind. Microbiol. Biotechnol. 42 (6), 851 - 866 10.1007/s10295-015-1615-8