Details zur Publikation
|DOI / URL||Link|
|Titel (primär)||Stable isotope labeling by amino acids in cell culture based proteomics reveals differences in protein abundances between spiral and coccoid forms of the gastric pathogen Helicobacter pylori|
|Autor||Müller, S.A.; Pernitzsch, S.R.; Haange, S.-B.; Uetz, P.; von Bergen, M.; Sharma, C.M.; Kalkhof, S.;|
|Journal / Serie||Journal of Proteomics|
|POF III (gesamt)||F11;|
|Keywords||Helicobacter pylori; SILAC; cell morphology; quantitative proteomics|
Helicobacter pylori (H. pylori) is a ε-proteobacterium that colonizes the stomach of about half of the world’s population. Persistent infections have been associated with several gastric diseases. Mainly rod- or spiral shaped but also coccoid forms have been isolated from mucus layer biopsies of patients. It is still being debated whether the coccoid form can be transformed back into the spiral form or whether this morphology is a result of bacterial cell death or persistence.
We established stable isotope labeling by amino acids in cell culture (SILAC) for quantitative proteomics of H. pylori and applied it to investigate differences between the spiral and the coccoid morphology. We detected 72% and were able to relatively quantify 47% of the H. pylori proteome.
Proteins involved in cell division, transcriptional and translational processes showed a lower abundance in coccoid cells. Additionally, proteins related to host colonization, including CagA, the arginase RocF, and the TNF-α inducing protein were down-regulated. The fact that outer membrane proteins were observed at higher abundances might represent a mechanism for immune evasion but also preserves adherence to host cells. The established protocol for relative protein quantification of H. pylori samples offers new possibilities for research on H. pylori.
Our study shows that SILAC can be employed to study protein abundance changes in H. pylori. We have chosen to establish SILAC for H. pylori because it facilitates fractionation on both, protein and peptide level and thus enables deep proteome coverage. Furthermore, SILAC allows robust and highly accurate protein quantification. The manuscript includes a detailed description of the applied method, suggestions for further improvement as well as a practical application.
The investigation of differences between the coccoid and infectious spiral morphology of H. pylori with SILAC revealed the regulation of protein groups that are involved in host colonization, mobility, cell division as well as transcriptional and translational processes. The provided data will help molecular biologist to focus on relevant pathways that were found to be regulated in response morphological changes.
Furthermore, the application of SILAC offers new possibilities to study the biology of H. pylori. It enables to monitor protein abundance changes in response to certain stimuli such as oxygen stress or antibiotics. Moreover, SILAC raises the possibility to study co-cultures of host cells and H. pylori on protein level. Additionally, pulsed SILAC experiments enable the quantification of protein turnover.
|Müller, S.A., Pernitzsch, S.R., Haange, S.-B., Uetz, P., von Bergen, M., Sharma, C.M., Kalkhof, S. (2015):
Stable isotope labeling by amino acids in cell culture based proteomics reveals differences in protein abundances between spiral and coccoid forms of the gastric pathogen Helicobacter pylori
J. Proteomics 126 , 34 - 45