Details zur Publikation |
Kategorie | Textpublikation |
Referenztyp | Berichte |
URL | R package version 1.2.0 http://www.bioconductor.org/packages/release/bioc/html/flowCyBar.html |
Titel (primär) | flowCyBar: Analyze flow cytometric data using gate information R package version 1.2.0 http://www.bioconductor.org/packages/release/bioc/html/flowCyBar.html October 2014: 1491 downloads (distinct IPs: December 2014: ~2000) |
Autor | Schumann, J.; Koch, C.; Günther, S.; Fetzer, I.; Müller, S. |
Erscheinungsjahr | 2014 |
Department | UMB |
Sprache | englisch |
Abstract | CyBar means cytometric barcoding and is a tool for evaluation of cytometric datasets by using gate information. On the rst hand it was developed for evaluation of microbial cytometric data but it can also be used for any other cytometric dataset. CyBar is used to record cell abundances per gate by creating a gate template that is valid for all samples that are part of an investigation. Thus, cell abundance variation per gate over time or per treatment is provided. As a result, the gate based population or community alterations are quantied and visualized in heatmaps. In addition, functional maps can be designed that indicate certain functions of cells in respective gates due to abiotic parameters that are recorded in parallel to the cytometric measurements. Also interactions of cells between gates can be suggested. The procedure allows a segregated and/or function-dependent identication of subpopulations/-communities of interest e.g. for cell sorting. The CyBar is useful to identify dynamics in a cell system, to nd factors that shape a population or community and to make sort decisions easier. |
dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15750 |
Schumann, J., Koch, C., Günther, S., Fetzer, I., Müller, S. (2014): flowCyBar: Analyze flow cytometric data using gate information R package version 1.2.0 http://www.bioconductor.org/packages/release/bioc/html/flowCyBar.html October 2014: 1491 downloads (distinct IPs: December 2014: ~2000) |