Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Mass spectrometric detection, identification, and fragmentation of arseno-phytochelatins
Autor Schmied-Tobies, M.I.H.; Arroyo-Abad, U.; Mattusch, J.; Reemtsma, T.;
Journal / Serie Journal of Mass Spectrometry
Erscheinungsjahr 2014
Department ANA;
Band/Volume 49
Heft 11
Sprache englisch;
POF III (gesamt) T42;
Keywords arsenic; complexation; analysis; plants; heavy metals; toxicity
UFZ Querschnittsthemen RU3;
Abstract Phytochelatins (PC) are cystein-rich oligopeptides in plants for coordination with toxic metals and metalloids via their thiol groups. The composition, structure, and mass spectrometric fragmentation of arseno-PC (As-PC) with PC of different degree of oligomerization (PC2–PC5) in solution were studied using liquid chromatography coupled in parallel to inductively coupled plasma mass spectrometry and electrospray ionization quadrupole time-of-flight mass spectrometry. As-PC were detected from As(PC2) to As(PC5) with an increasing number of isomers that differ in the position of thiol groups bound to As. Thermodynamic modeling supported the identification process in case of these isomers. Mass spectrometric fragmentation of the As-PC does not follow the established pattern of peptides but is governed by the formation of series of As-containing annular cations, which coordinate to As via S, N, or O. Structure proposals for 30 As-PC fragment ions in the range m/z 147.92 to m/z 1290.18 are elaborated. Many of these fragment ions are characteristic to several As-PC and may be suited for a screening for As-PC in plant extracts. The mass spectrometric data offer the perspective for a future more sensitive determination of As-PC by means of liquid chromatography tandem mass spectrometry with multiple reaction monitoring
ID 15647
dauerhafte UFZ-Verlinkung
Schmied-Tobies, M.I.H., Arroyo-Abad, U., Mattusch, J., Reemtsma, T. (2014):
Mass spectrometric detection, identification, and fragmentation of arseno-phytochelatins
J. Mass Spectrom. 49 (11), 1148 - 1155