Details zur Publikation

Referenztyp Buchkapitel
DOI / URL Link
Titel (primär) Advanced techniques for in situ analysis of the biofilm matrix (structure, composition, dynamics) by means of laser scanning microscopy
Titel (sekundär) Microbial biofilms: methods and protocols
Autor Neu, T.R.; Lawrence, J.R.;
Herausgeber Donelli, G.;
Journal / Serie Methods in Molecular Biology
Erscheinungsjahr 2014
Department FLOEK;
Band/Volume 1147
Sprache englisch;
POF III (gesamt) T34;
Keywords Biofilm; Bioaggregate; Biofilm matrix; Extracellular polymeric substances (EPS); Confocal laser scanning microscopy (CLSM); Laser scanning microscopy (LSM); Fluorescence; Fluorescence lectin-binding analysis (FLBA)
UFZ Querschnittsthemen RU2;
Abstract The extracellular constituents in bioaggregates and biofi lms can be imaged four dimensionally by using
laser scanning microscopy. In this protocol we provide guidance on how to examine the various extracellular
compartments in between microbial cells and communities associated with interfaces. The current
options for fl uorescence staining of matrix compounds and extracellular microhabitats are presented.
Furthermore, practical aspects are discussed and useful notes are added. The chapter ends with a brief
introduction to other approaches for EPS analysis and an outlook for future needs.
ID 15572
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15572
Neu, T.R., Lawrence, J.R. (2014):
Advanced techniques for in situ analysis of the biofilm matrix (structure, composition, dynamics) by means of laser scanning microscopy
In: Donelli, G. (ed.)
Microbial biofilms: methods and protocols
Methods in Molecular Biology 1147
Humana Press, p. 43 - 64