Details zur Publikation
Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.envpol.2014.10.003
Titel (primär) Metabolic transformation as a diagnostic tool for the selection of candidate promutagens in effect-directed analysis
Autor Hug, C.; Krauss, M. ORCID logo ; Nüsser, L.; Hollert, H.; Brack, W.
Quelle Environmental Pollution
Erscheinungsjahr 2015
Department WANA
Band/Volume 196
Seite von 114
Seite bis 124
Sprache englisch
Keywords Ames fluctuation test; Effect-directed analysis; Candidate exclusion; Liquid chromatography-high resolution mass spectrometry (LC-HRMS)
UFZ Querschnittsthemen RU3;
Abstract To implement metabolic activation by S9 rat liver homogenate in the selection of candidate promutagens in effect-directed analysis, we critically assessed the capability of LC-HRMS measurements to detect depletion and formation of metabolites by S9 exposure. The exposure of a reference mixture to S9 led to a depletion by >70% for most compounds. Other processes than metabolism were excluded as significant contribution to compound depletion. Metabolites formed by S9 exposure were identified and S9 metabolism was incorporated in the identification of candidate promutagens in a wastewater treatment plant (WWTP) effluent with mutagenic activity only after metabolic activation by S9. The metabolism by S9 in the WWTP effluent was confirmed. Based on a candidate exclusion of all peaks not depleted, thus not activated by the S9 mix, the number of candidate promutagens was reduced by 40%. Selected remaining candidates were evaluated and identified, but could not be confirmed as promutagens.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15447
Hug, C., Krauss, M., Nüsser, L., Hollert, H., Brack, W. (2015):
Metabolic transformation as a diagnostic tool for the selection of candidate promutagens in effect-directed analysis
Environ. Pollut. 196 , 114 - 124 10.1016/j.envpol.2014.10.003