Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1007/s00253-014-6037-4
Volltext Shareable Link
Titel (primär) Methods for visualising active microbial toluene and benzene degraders in in situ microcosms
Autor Schurig, C.; Müller, C.W.; Höschen, C.; Prager, A.; Kothe, E.; Beck, H.; Miltner, A. ORCID logo ; Kästner, M.
Journal / Serie Applied Microbiology and Biotechnology
Erscheinungsjahr 2015
Department UBT
Band/Volume 99
Heft 2
Seite von 957
Seite bis 968
Sprache englisch
Keywords NanoSIMS; 13C; Microbial activity; In situ microcosms ; DP-BACTRAPs; Benzene
UFZ Querschnittsthemen RU3;
Abstract Natural attenuation maybe a cost-efficient option for bioremediation of contaminated sites but requires knowledge about the activity of degrading microbes under in situ conditions. In order to link microbial activity to the spatial distribution of contaminant degraders, we combined the recently improved in situ microcosm approach, so-called ‘direct-push bacterial trap’ (DP-BACTRAP), with nano-scale secondary ion mass spectrometry (NanoSIMS) analysis on samples from contaminated constructed wetlands. This approach is based on initially sterile microcosms amended with 13C-labelled benzene as a source of carbon and energy for microorganisms. The microcosms were introduced directly in the constructed wetland, where they were colonised by indigenous microorganisms from the sediment. After incubation in the field, the samples were analysed by NanoSIMS, scanning electron microscopy (SEM) and fluorescence microscopy in order to visualise 13C-labelled microbial biomass on undisturbed samples from the microcosms. With the approach developed, we successfully visualised benzene-degrading microbes on solid materials with high surface area by means of NanoSIMS. Moreover, we could demonstrate the feasibility of NanoSIMS analysis of unembedded porous media with a highly complex topography, which was frequently reasoned to not lead to sufficient results.
dauerhafte UFZ-Verlinkung
Schurig, C., Müller, C.W., Höschen, C., Prager, A., Kothe, E., Beck, H., Miltner, A., Kästner, M. (2015):
Methods for visualising active microbial toluene and benzene degraders in in situ microcosms
Appl. Microbiol. Biotechnol. 99 (2), 957 - 968