Details zur Publikation

Kategorie Textpublikation
Referenztyp Buchkapitel
DOI 10.1007/8623_2014_17
Titel (primär) Isolation and analysis of membrane phospholipids as biomarkers
Titel (sekundär) Hydrocarbon and lipid microbiology protocols. Biochemical methods
Autor Hachicho, N.; Heipieper, H.J. ORCID logo
Herausgeber McGenity, T.J.; Timmis, K.N.; Nogales, B.
Quelle Springer Protocols Handbooks
Erscheinungsjahr 2016
Department UBT
Seite von 123
Seite bis 132
Sprache englisch
Keywords Biodiversity; Biomarkers; Community analysis; Microbial ecology; Phospholipid fatty acids (PLFA); Stress response
UFZ Querschnittsthemen RU3;
Abstract The protocol describes the analysis and data interpretation of membrane phospholipid fatty acid (PLFA) profiles as biomarkers for the characterisation of microbial communities in environmental samples, with special emphasis on soils and sediments contaminated with organic hydrocarbons. This simple method in microbial ecology studies provides an estimate of the viable microbial biomass, first indications of the structure of the microbial community, as well as the physiological status of the microbiota. In environmental biotechnological applications, PLFA analysis is a useful tool for monitoring bioremediation and allows direct validation of existing biological activities as well as hints for shifts in the microbiota caused by successful biological treatment strategies.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15278
Hachicho, N., Heipieper, H.J. (2016):
Isolation and analysis of membrane phospholipids as biomarkers
In: McGenity, T.J., Timmis, K.N., Nogales, B. (eds.)
Hydrocarbon and lipid microbiology protocols. Biochemical methods
Springer Protocols Handbooks
Springer, Berlin, Heidelberg, p. 123 - 132 10.1007/8623_2014_17