Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1111/1462-2920.12596 |
| Titel (primär) | Genome and physiology of the ascomycete filamentous fungus Xeromyces bisporus, the most xerophilic organism isolated to date |
| Autor | Leong, S.L.; Lantz, H.; Pettersson, O.V.; Frisvad, J.C.; Thrane, U.; Heipieper, H.J.
; Dijksterhuis, J.; Grabherr, M.; Pettersson, M.; Tellgren-Roth, C.; Schnürer, J. |
| Quelle | Environmental Microbiology |
| Erscheinungsjahr | 2015 |
| Department | UBT |
| Band/Volume | 17 |
| Heft | 2 |
| Seite von | 496 |
| Seite bis | 513 |
| Sprache | englisch |
| Supplements | Supplement 1 Supplement 2 Supplement 3 Supplement 4 Supplement 5 |
| UFZ Querschnittsthemen | RU3; |
| Abstract | Xeromyces bisporus can grow on sugary substrates down to 0.61, an extremely low water activity. Its genome size is approx. 22Mb. Gene clusters encoding for secondary metabolites were conspicuously absent; secondary metabolites were not detected experimentally. Thus, in its ‘dry’ but nutrient-rich environment, X. bisporus appears to have relinquished abilities for combative interactions. Elements to sense / signal osmotic stress, e.g. HogA pathway, were present in X. bisporus. However, transcriptomes at optimal (∼0.89) vs low aw (0.68) revealed differential expression of only a few stress-related genes; among these, certain (not all) steps for glycerol synthesis were upregulated. X. bisporus increased glycerol production during hypo- and hyper-osmotic stress, and much of its wet weight comprised water and rinsable solutes; leaked solutes may form a protective slime. X. bisporus and other food-borne moulds increased membrane fatty acid saturation as water activity decreased. Such modifications did not appear to be transcriptionally regulated in X. bisporus; however, genes modulating sterols, phospholipids and the cell-wall were differentially expressed. X. bisporus was previously proposed to be a ‘chaophile’, preferring solutes that disorder biomolecular structures. Both X. bisporus and the closely related xerophile, Xerochrysium xerophilum, with low membrane unsaturation indices, could represent a phylogenetic cluster of ‘chaophiles’. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15277 |
| Leong, S.L., Lantz, H., Pettersson, O.V., Frisvad, J.C., Thrane, U., Heipieper, H.J., Dijksterhuis, J., Grabherr, M., Pettersson, M., Tellgren-Roth, C., Schnürer, J. (2015): Genome and physiology of the ascomycete filamentous fungus Xeromyces bisporus, the most xerophilic organism isolated to date Environ. Microbiol. 17 (2), 496 - 513 10.1111/1462-2920.12596 |
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