Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
URL http://www.jspb.ru/issues/2013/N4/JSPB_2013_4_345-351.html
Titel (primär) A comparative analysis of hsp70 mRNA and HSP70 protein expression in two distant populations of the Baikal amphipod Eulimnogammarus verrucosus (Gerstf., 1858)
Autor Madyarova, E.V.; Lubyaga, J.A.; Gurkov, A.N.; Vereshchagina, K.P.; Kondrateva, E.M.; Shchapova, E.P.; Timofeyev, M.A.; Luckenbach, T. ORCID logo ; Bedulina, D.S.
Quelle Journal of Stress Physiology & Biochemistry
Erscheinungsjahr 2013
Department BIOTOX
Band/Volume 9
Heft 4
Seite von 345
Seite bis 351
Sprache russisch
Keywords Amphipods; Baikal; Eulimnogammarus verrucosus; gene expression; hsp70
UFZ Querschnittsthemen RU2;
Abstract Basal levels of  hsp70 (mRNA) and HSP70 (protein) were measured in representatives from two distant populations of the amphipod Eulimnogammarus verrucosus (Gerstf.) endemic to Lake Baikal using quantitative PCR (qPCR) and Western blotting upon lab acclimation. In addition, hsp70 mRNA and HSP70 protein levels in animals subjected to gradual temperature increase were measured. In contrast to  hsp70 mRNA levels that were similar in both populations basal HSP70 protein levels were  higher  in  representatives  of  the  southern  population  than  compared  to  the  northern population.  Changes  in  hsp70 mRNA  levels  at  increased  temperature  tended  to  be  more pronounced in the southern population compared to the northern one, whereas HSP70 protein changed only slightly in both populations. The data indicate differences in the phenotypic plasticity of the hsp/HSP70 stress response in different populations of this species.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15203
Madyarova, E.V., Lubyaga, J.A., Gurkov, A.N., Vereshchagina, K.P., Kondrateva, E.M., Shchapova, E.P., Timofeyev, M.A., Luckenbach, T., Bedulina, D.S. (2013):
A comparative analysis of hsp70 mRNA and HSP70 protein expression in two distant populations of the Baikal amphipod Eulimnogammarus verrucosus (Gerstf., 1858)
Journal of Stress Physiology & Biochemistry 9 (4), 345 - 351