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Titel (primär) Quantification of Al2O3 nanoparticles in human cell lines applying inductively coupled plasma mass spectrometry (neb-ICP-MS, LA-ICP-MS) and flow cytometry-based methods
Autor Böhme, S.; Stärk, H.-J.; Meißner, T.; Springer, A.; Reemtsma, T.; Kühnel, D.; Busch, W.;
Journal / Serie Journal of Nanoparticle Research
Erscheinungsjahr 2014
Department BIOTOX; ANA;
Band/Volume 16
Sprache englisch;
POF III (gesamt) T42;
Keywords inductively coupled plasma mass spectrometry (ICP-MS); Flow cytometry; Size dependency; Cellular internalization; Aluminum oxide
UFZ Querschnittsthemen RU3;
Abstract In order to quantify and compare the uptake of aluminum oxide nanoparticles of three different sizes into two human cell lines (skin keratinocytes (HaCaT) and lung epithelial cells (A549)), three analytical methods were applied: digestion followed by nebulization inductively coupled plasma mass spectrometry (neb-ICP-MS), direct laser ablation ICP-MS (LA-ICP-MS), and flow cytometry. Light and electron microscopy revealed an accumulation and agglomeration of all particle types within the cell cytoplasm, whereas no particles were detected in the cell nuclei. The internalized Al2O3 particles exerted no toxicity in the two cell lines after 24 h of exposure. The smallest particles with a primary particle size (x BET) of 14 nm (Alu1) showed the lowest sedimentation velocity within the cell culture media, but were calculated to have settled completely after 20 h. Alu2 (x BET = 111 nm) and Alu3 (x BET = 750 nm) were calculated to reach the cell surface after 7 h and 3 min, respectively. The internal concentrations determined with the different methods lay in a comparable range of 2–8 µg Al2O3/cm2 cell layer, indicating the suitability of all methods to quantify the nanoparticle uptake. Nevertheless, particle size limitations of analytical methods using optical devices were demonstrated for LA-ICP-MS and flow cytometry. Furthermore, the consideration and comparison of particle properties as parameters for particle internalization revealed the particle size and the exposure concentration as determining factors for particle uptake.
ID 15153
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15153
Böhme, S., Stärk, H.-J., Meißner, T., Springer, A., Reemtsma, T., Kühnel, D., Busch, W. (2014):
Quantification of Al2O3 nanoparticles in human cell lines applying inductively coupled plasma mass spectrometry (neb-ICP-MS, LA-ICP-MS) and flow cytometry-based methods
J. Nanopart. Res. 16 , 2592