Details zur Publikation |
Kategorie | Textpublikation |
Referenztyp | Zeitschriften |
DOI | 10.1021/es500004z |
Titel (primär) | Experimental results and integrated modelling of bacterial growth on insoluble hydrophobic substrate (phenanthrene) |
Autor | Adam, I.K.U.; Rein, A.; Miltner, A. ; Fulgêncio, A.C.D.; Trapp, S.; Kästner, M. |
Quelle | Environmental Science & Technology |
Erscheinungsjahr | 2014 |
Department | UBT |
Band/Volume | 48 |
Heft | 15 |
Seite von | 8717 |
Seite bis | 8726 |
Sprache | englisch |
UFZ Querschnittsthemen | RU3; |
Abstract | Metabolism of a low-soluble substrate is limited by dissolution and availability and can hardly be determined. We developed a numerical model simultaneously calculating dissolution kinetics of such substrates, its metabolism and microbial growth (Monod kinetics with decay) and tested it with three aerobic phenanthrene (PHE) degraders: Novosphingobium pentaromativorans US6-1, Sphingomonas sp. EPA505 and Sphingobium yanoikuyae B1. PHE was present as microcrystals, providing non-limiting conditions for growth. Total PHE and protein concentration were tracked over 6 to 12 days. The model was fitted to the test results for the rates of dissolution, metabolism and growth. The strains showed similar efficiency, with vmax values of 12 to 18 g dw g-1 d-1, yields of 0.21 g g-1, maximum growth rates of 2.5 to 3.8 d-1 and decay rates of 0.04 to 0.05 d-1. Sensitivity analysis with the model shows that i) retention in crystals, NAPLs or by sequestration compete with biodegradation, ii) bacterial growth conditions (dissolution flux and resulting chemical activity of substrate) are more relevant for the final state of the system than the initial biomass; and iii) the desorption flux regulates the turnover in the presence of solid-state, sequestered (aged) or NAPL substrate sources. |
dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15020 |
Adam, I.K.U., Rein, A., Miltner, A., Fulgêncio, A.C.D., Trapp, S., Kästner, M. (2014): Experimental results and integrated modelling of bacterial growth on insoluble hydrophobic substrate (phenanthrene) Environ. Sci. Technol. 48 (15), 8717 - 8726 10.1021/es500004z |