Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Metabolomic biomarkers correlating with hepatic lipidosis in dairy cows
Autor Imhasly, S.; Naegeli, H.; Baumann, S.; von Bergen, M.; Luch, A.; Jungnickel, H.; Potratz, S.; Gerspach, C.;
Journal / Serie BMC Veterinary Research
Erscheinungsjahr 2014
Department METABOX; PROTEOM;
Band/Volume 10
Sprache englisch;
POF III (gesamt) F11;
Keywords Biomarker; Fatty liver; Lipidosis; Metabolomics
UFZ Querschnittsthemen RU3;
Abstract

Background

Hepatic lipidosis or fatty liver disease is a major metabolic disorder of high-producing dairy cows that compromises animal performance and, hence, causes heavy economic losses worldwide. This syndrome, occurring during the critical transition from gestation to early lactation, leads to an impaired health status, decreased milk yield, reduced fertility and shortened lifetime. Because the prevailing clinical chemistry parameters indicate advanced liver damage independently of the underlying disease, currently, hepatic lipidosis can only be ascertained by liver biopsy. We hypothesized that the condition of fatty liver disease may be accompanied by an altered profile of endogenous metabolites in the blood of affected animals.

Results

To identify potential small-molecule biomarkers as a novel diagnostic alternative, the serum samples of diseased dairy cows were subjected to a targeted metabolomics screen by triple quadrupole mass spectrometry. A subsequent multivariate test involving principal component and linear discriminant analyses yielded 29 metabolites (amino acids, phosphatidylcholines and sphingomyelines) that, in conjunction, were able to distinguish between dairy cows with no hepatic lipidosis and those displaying different stages of the disorder.

Conclusions

This proof-of-concept study indicates that metabolomic profiles, including both amino acids and lipids, distinguish hepatic lipidosis from other peripartal disorders and, hence, provide a promising new tool for the diagnosis of hepatic lipidosis. By generating insights into the molecular pathogenesis of hepatic lipidosis, metabolomics studies may also facilitate the prevention of this syndrome.

ID 14899
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=14899
Imhasly, S., Naegeli, H., Baumann, S., von Bergen, M., Luch, A., Jungnickel, H., Potratz, S., Gerspach, C. (2014):
Metabolomic biomarkers correlating with hepatic lipidosis in dairy cows
BMC Vet. Res. 10 , art. 122