Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1111/1574-6968.12429
Titel (primär) A PCR-based assay for the detection of anaerobic naphthalene degradation
Autor Morris, B.E.L.; Gissibl, A.; Kümmel, S.; Richnow, H.-H.; Boll, M.
Quelle FEMS Microbiology Letters
Erscheinungsjahr 2014
Department ISOBIO
Band/Volume 354
Heft 1
Seite von 55
Seite bis 59
Sprache englisch
Keywords polycyclic aromatic hydrocarbons; PCR-based assay; anaerobic aromatic degradation; 2-naphthoyl-CoA reductase
UFZ Querschnittsthemen RU3;
Abstract Polycyclic aromatic hydrocarbons (PAH) are widespread environmental pollutants of considerable risk to human health. The aerobic degradation of PAH via oxygenase reactions has been studied for several decades. In contrast, it was not until very recent that the first key enzyme involved in anaerobic PAH degradation, the dearomatizing 2-naphthoyl-CoA reductase, was isolated and characterized. In this work, a PCR-based functional assay was developed to detect microorganisms that have the ability to anaerobically degrade naphthalene, as a model for larger PAH. The degenerative oligonucleotide probes introduced here amplified a highly conserved region of the gene encoding 2-naphthoyl-CoA reductase (Ncr) in numerous sulfate-reducing pure cultures and environmental enrichments. The assay provides the first molecular tool for monitoring the anaerobic degradation of a model PAH.
dauerhafte UFZ-Verlinkung
Morris, B.E.L., Gissibl, A., Kümmel, S., Richnow, H.-H., Boll, M. (2014):
A PCR-based assay for the detection of anaerobic naphthalene degradation
FEMS Microbiol. Lett. 354 (1), 55 - 59 10.1111/1574-6968.12429