Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.scitotenv.2013.07.073
Titel (primär) Physiological responses to nanoCuO in fungi from non-polluted and metal-polluted streams
Autor Pradhan, A.; Seena, S.; Dobritzsch, D.; Helm, S.; Gerth, K.; Dobritzsch, M.; Krauss, G.-J.; Schlosser, D. ORCID logo ; Pascoal, C.; Cássio, F.
Quelle Science of the Total Environment
Erscheinungsjahr 2014
Department UMB
Band/Volume 466–467
Seite von 556
Seite bis 563
Sprache englisch
Keywords Keywords NanoCuO; Fungal biomass; Mycelial morphology; Biosorption; Laccases
UFZ Querschnittsthemen RU3;
Abstract Nanocopper oxide (nanoCuO) is among the most widely used metal oxide nanoparticles which increases their chance of being released into freshwaters. Fungi are the major microbial decomposers of plant litter in streams. Fungal laccases are multicopper oxidase enzymes that are involved in the degradation of lignin and various xenobiotic compounds. We investigated the effects of nanoCuO (5 levels, ≤ 200 mg L− 1) on four fungal isolates collected from metal-polluted and non-polluted streams by analyzing biomass production, changes in mycelial morphology, laccase activity, and quantifying copper adsorbed to mycelia, and ionic and nanoparticulate copper in the growth media. The exposure to nanoCuO decreased the biomass produced by all fungi in a concentration- and time-dependent manner. Inhibition of biomass production was stronger in fungi from non-polluted (EC50(10 days) ≤ 31 mg L− 1) than from metal-polluted streams (EC50(10 days) ≥ 65.2 mg L− 1). NanoCuO exposure led to cell shrinkage and mycelial degeneration, particularly in fungi collected from non-polluted streams. Adsorption of nanoCuO to fungal mycelia increased with the concentration of nanoCuO in the medium and was higher in fungi from non-polluted streams. Extracellular laccase activity was induced by nanoCuO in two fungal isolates in a concentration-dependent manner, and was highly correlated with adsorbed Cu and/or ionic Cu released by dissolution from nanoCuO. Putative laccase gene fragments were also detected in these fungi. Lack of substantial laccase activity in the other fungal isolates was corroborated by the absence of laccase-like gene fragments.
dauerhafte UFZ-Verlinkung
Pradhan, A., Seena, S., Dobritzsch, D., Helm, S., Gerth, K., Dobritzsch, M., Krauss, G.-J., Schlosser, D., Pascoal, C., Cássio, F. (2014):
Physiological responses to nanoCuO in fungi from non-polluted and metal-polluted streams
Sci. Total Environ. 466–467 , 556 - 563 10.1016/j.scitotenv.2013.07.073