Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.3390/s140203737
Titel (primär) DNA-aptamers binding aminoglycoside antibiotics
Autor Nikolaus, N.; Strehlitz, B.
Quelle Sensors
Erscheinungsjahr 2014
Department UBZ
Band/Volume 14
Heft 2
Seite von 3737
Seite bis 3755
Sprache englisch
Keywords aptamer; Capture-SELEX; aminoglycoside antibiotic; kanamycin
UFZ Querschnittsthemen RU2;
Abstract Aptamers are short, single stranded DNA or RNA oligonucleotides that are able to bind specifically and with high affinity to their non-nucleic acid target molecules. This binding reaction enables their application as biorecognition elements in biosensors and assays. As antibiotic residues pose a problem contributing to the emergence of antibiotic-resistant pathogens and thereby reducing the effectiveness of the drug to fight human infections, we selected aptamers targeted against the aminoglycoside antibiotic kanamycin A with the aim of constructing a robust and functional assay that can be used for water analysis. With this work we show that aptamers that were derived from a Capture-SELEX procedure targeting against kanamycin A also display binding to related aminoglycoside antibiotics. The binding patterns differ among all tested aptamers so that there are highly substance specific aptamers and more group specific aptamers binding to a different variety of aminoglycoside antibiotics. Also the region of the aminoglycoside antibiotics responsible for aptamer binding can be estimated. Affinities of the different aptamers for their target substance, kanamycin A, are measured with different approaches and are in the micromolar range. Finally, the proof of principle of an assay for detection of kanamycin A in a real water sample is given.
dauerhafte UFZ-Verlinkung
Nikolaus, N., Strehlitz, B. (2014):
DNA-aptamers binding aminoglycoside antibiotics
Sensors 14 (2), 3737 - 3755 10.3390/s140203737