Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Buchkapitel |
| DOI | 10.1007/978-1-4939-0467-9_19 |
| Titel (primär) | Chip calorimetry for evaluation of biofilm treatment with biocides, antibiotics, and biological agents |
| Titel (sekundär) | Microbial biofilms: methods and protocols |
| Autor | Morais, F.M.; Buchholz, F.; Maskow, T.
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| Herausgeber | Donelli, G. |
| Quelle | Methods in Molecular Biology |
| Erscheinungsjahr | 2014 |
| Department | UMB |
| Band/Volume | 1147 |
| Seite von | 267 |
| Seite bis | 275 |
| Sprache | englisch |
| Keywords | Calorimetry; Antibiotic; Biocides; Antimicrobial agents; Activity measurement; Susceptibility testing; Noninvasive; Realtime monitoring |
| UFZ Querschnittsthemen | RU3; |
| Abstract | Any growth or bioconversion in biofilms is accompanied by the release of heat. The heat (in J) is tightly related to the stoichiometry of the respective process via law of Hess, and the heat production rate (in W or J/s) is additionally related to the process kinetics. This heat and the heat production rate can nowadays be measured by modern calorimetry with extremely high sensitivity. Flow-through calorimetry allows the measurement of bioprocesses in biofilms in real time, without the need of invasive sample preparation and disturbing of biofilm processes. Furthermore, it can be applied for long-term measurements and is even applicable to turbid media. Chip or miniaturized calorimeters have the additional advantages of extremely short thermal equilibration times and the requirement of very small amounts of media and chemicals. The precision of flow-through chip calorimeters (about 3 mW/L) allows the detection of early stages of biofilm development (about 105 bacteria cm−2). |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=14485 |
| Morais, F.M., Buchholz, F., Maskow, T. (2014): Chip calorimetry for evaluation of biofilm treatment with biocides, antibiotics, and biological agents In: Donelli, G. (ed.) Microbial biofilms: methods and protocols Methods in Molecular Biology 1147 Springer Nature, p. 267 - 275 10.1007/978-1-4939-0467-9_19 |
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