Details zur Publikation

Referenztyp Buchkapitel
DOI / URL Link
Titel (primär) Chip calorimetry for evaluation of biofilm treatment with biocides, antibiotics, and biological agents
Titel (sekundär) Microbial biofilms: methods and protocols
Autor Morais, F.M.; Buchholz, F.; Maskow, T.;
Herausgeber Donelli, G.;
Journal / Serie Methods in Molecular Biology
Erscheinungsjahr 2014
Department UMB;
Band/Volume 1147
Sprache englisch;
POF III (gesamt) T41;
Keywords Calorimetry; Antibiotic; Biocides; Antimicrobial agents; Activity measurement; Susceptibility testing; Noninvasive; Realtime monitoring
UFZ Querschnittsthemen RU3;
Abstract Any growth or bioconversion in biofilms is accompanied by the release of heat. The heat (in J) is tightly related to the stoichiometry of the respective process via law of Hess, and the heat production rate (in W or J/s) is additionally related to the process kinetics. This heat and the heat production rate can nowadays be measured by modern calorimetry with extremely high sensitivity. Flow-through calorimetry allows the measurement of bioprocesses in biofilms in real time, without the need of invasive sample preparation and disturbing of biofilm processes. Furthermore, it can be applied for long-term measurements and is even applicable to turbid media. Chip or miniaturized calorimeters have the additional advantages of extremely short thermal equilibration times and the requirement of very small amounts of media and chemicals. The precision of flow-through chip calorimeters (about 3 mW/L) allows the detection of early stages of biofilm development (about 105 bacteria
cm−2).
ID 14485
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=14485
Morais, F.M., Buchholz, F., Maskow, T. (2014):
Chip calorimetry for evaluation of biofilm treatment with biocides, antibiotics, and biological agents
In: Donelli, G. (ed.)
Microbial biofilms: methods and protocols
Methods in Molecular Biology 1147
Humana Press, p. 267 - 275