Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) High-yield production of aromatic peroxygenase by the agaric fungus Marasmius rotula
Autor Gröbe, G.; Ullrich, R.; Pecyna, M.J.; Kapturska, D.; Friedrich, S.; Hofrichter, M.; Scheibner, K.;
Journal / Serie AMB Express
Erscheinungsjahr 2011
Department UMB; BOOEK;
Band/Volume 1
Sprache englisch;
Keywords Peroxidase; Basidiomycota; Cytochrome P450; Bioreactor
Abstract An extracellular peroxygenase from Marasmius rotula was produced in liquid culture, chromatographically purified and partially characterized. This is the third aromatic peroxygenase (APO) that has been characterized in detail and the first one that can be produced in high yields. The highest enzyme levels of about 41,000 U l-1 (corresponding to appr. 445 mg l-1 APO protein) exceeded the hitherto reported levels more than 40-fold and were detected in carbon- and nitrogen-rich complex media. The enzyme was purified by FPLC to apparent homogeneity (SDS-PAGE) with a molecular mass of 32 kDa (27 kDa after deglycosylation) and isoelectric points between 4.97 and 5.27. The UV-visible spectrum of the native enzyme showed a characteristic maximum (Soret band) at 418 nm that shifted after reduction with sodium dithionite and flushing with carbon monoxide to 443 nm. The pH optimum of the M. rotula enzyme was found to vary between pH 5 and 6 for most reactions studied. The apparent Km-values for 2,6-dimethoxyphenol, benzyl alcohol, veratryl alcohol, naphthalene and H2O2 were 0.133, 0.118, 0.279, 0.791 and 3.14 mM, respectively. M. rotula APO was found to be highly stable in a pH range from 5 to 10 as well as in the presence of organic solvents (50% vol/vol) such as methanol, acetonitrile and N,N-dimethylformamide. Unlike other APOs, the peroxygenase of M. rotula showed neither brominating nor chlorinating activities. 
ID 14439
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=14439
Gröbe, G., Ullrich, R., Pecyna, M.J., Kapturska, D., Friedrich, S., Hofrichter, M., Scheibner, K. (2011):
High-yield production of aromatic peroxygenase by the agaric fungus Marasmius rotula
AMB Express 1 , art. 31