Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Sustainability of industrial yeast serial repitching practice studied by gene expression and correlation analysis
Autor Bühligen, F.; Rüdinger, P.; Fetzer, I.; Stahl, F.; Scheper, T.; Harms, H.; Müller, S.;
Journal / Serie Journal of Biotechnology
Erscheinungsjahr 2013
Department UMB;
Band/Volume 168
Heft 4
Sprache englisch;
POF III (gesamt) R31; T41;
Keywords Saccharomyces pastorianus; Serial repitching; Transcriptome; Stress; Process optimization
UFZ Querschnittsthemen RU4;
Abstract

Bottom-fermenting Saccharomyces pastorianus strains driving brewing fermentation processes are usually reused several times. It is still unclear, whether the number of successions may have an impact on cell physiology prompting consequences for brewing quality. In this study, fermentation performance of up to twenty consecutive runs in a brewery was investigated. For each run mRNA expression levels of cellular marker molecules, which are known to correlate with metabolism, hexose transport, aging processes, stress response mechanisms and flocculation capability was estimated to obtain information on changes in cell physiology over the successive runs. Low-density microarrays were used for this purpose and the resulting gene expression profiles were finally correlated with changes in the abiotic micro-environments. A surprising stability of the marker molecule expression profiles within each specific serial repitching was stated. Loss of flocculation or an advanced aging could not be detected during serial repitching in the analyzed brewery. However, certain runs of the serial repitchings showed high variation in stress response which was found to be caused by perturbations of the abiotic conditions. Regardless, the study showed that S. pastorianus can be used repeatedly in serial repitching processes without loss of prominent physiological characteristics.

ID 14316
dauerhafte UFZ-Verlinkung http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=14316
Bühligen, F., Rüdinger, P., Fetzer, I., Stahl, F., Scheper, T., Harms, H., Müller, S. (2013):
Sustainability of industrial yeast serial repitching practice studied by gene expression and correlation analysis
J. Biotechnol. 168 (4), 718 - 728