Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1104/pp.113.224303
Titel (primär) Speciation and distribution of arsenic in the nonhyperaccumulator macrophyte Ceratophyllum demersum
Autor Mishra, S.; Wellenreuther, G.; Mattusch, J.; Stärk, H.-J.; Küpper, H.
Journal / Serie Plant Physiology
Erscheinungsjahr 2013
Department ANA
Band/Volume 163
Heft 3
Seite von 1396
Seite bis 1408
Sprache englisch
UFZ Querschnittsthemen RU4;

Although arsenic (As) is a common pollutant worldwide, many questions about As metabolism in nonhyperaccumulator plants remain. Concentration- and tissue-dependent speciation and distribution of As was analyzed in the aquatic plant Ceratophyllum demersum to understand As metabolism in nonhyperaccumulator plants. Speciation was analyzed chromatographically (high-performance liquid chromatography-[inductively coupled plasma-mass spectrometry]-[electrospray ionization-mass spectrometry]) in whole-plant extracts and by tissue-resolution confocal x-ray absorption near-edge spectroscopy in intact shock-frozen hydrated leaves, which were also used for analyzing cellular element distribution through x-ray fluorescence. Chromatography revealed up to 20 As-containing species binding more than 60% of accumulated As. Of these, eight were identified as thiol-bound (phytochelatins [PCs], glutathione, and cysteine) species, including three newly identified complexes: Cys-As(III)-PC2, Cys-As-(GS)2, and GS-As(III)-desgly-PC2. Confocal x-ray absorption near-edge spectroscopy showed arsenate, arsenite, As-(GS)3, and As-PCs with varying ratios in various tissues. The epidermis of mature leaves contained the highest proportion of thiol (mostly PC)-bound As, while in younger leaves, a lower proportion of As was thiol bound. At higher As concentrations, the percentage of unbound arsenite increased in the vein and mesophyll of young mature leaves. At the same time, x-ray fluorescence showed an increase of total As in the vein and mesophyll but not in the epidermis of young mature leaves, while this was reversed for zinc distribution. Thus, As toxicity was correlated with a change in As distribution pattern and As species rather than a general increase in many tissues.

dauerhafte UFZ-Verlinkung
Mishra, S., Wellenreuther, G., Mattusch, J., Stärk, H.-J., Küpper, H. (2013):
Speciation and distribution of arsenic in the nonhyperaccumulator macrophyte Ceratophyllum demersum
Plant Physiol. 163 (3), 1396 - 1408