Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Alkane-degrading bacteria at the soil–litter interface: comparing isolates with T-RFLP-based community profiles
Autor Giebler, J.; Wick, L.Y.; Chatzinotas, A.; Harms, H.;
Journal / Serie FEMS Microbiology Ecology
Erscheinungsjahr 2013
Department UMB;
Band/Volume 86
Heft 1
Sprache englisch;
POF III (gesamt) T41;
Keywords soil alkane degradation potential;cultivation;growth matrix-based enrichment;alkB T-RFLP;seed bank;rare biosphere
UFZ Querschnittsthemen ru3
Abstract

Alkane-degrading bacteria were isolated from uncontaminated soil microcosms, which had been incubated with maize litter as natural alkane source. The isolates served to understand spatio-temporal community changes at the soil–litter interface, which had been detected using alkB as a functional marker gene for bacterial alkane degraders. To obtain a large spectrum of isolates, liquid subcultivation was combined with a matrix-assisted enrichment (Teflon membranes, litter). Elevated cell numbers of alkane degraders were detected by most probable number counting indicating enhanced alkane degradation potential in soil in response to litter treatment. Partial 16S rRNA gene sequencing of 395 isolates revealed forty different phylogenetic groups [operational taxonomic units (OTUs)] and spatio-temporal shifts in community composition. Ten OTUs comprised so far unknown alkane degraders, and five OTUs represented putative new bacterial genera. The combination of enrichment methods yielded a higher diversity of isolates than liquid subcultivation alone. Comparison of 16S rRNA gene T-RFLP profiles indicated that many alkane degraders present in the enrichments were not detectable in the DNA extracts from soil microcosms. These possibly rare specialists might represent a seed bank for the alkane degradation capacity in uncontaminated soil. This relevant ecosystem function can be fostered by the formation of the soil–litter interface.

ID 14114
dauerhafte UFZ-Verlinkung http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=14114
Giebler, J., Wick, L.Y., Chatzinotas, A., Harms, H. (2013):
Alkane-degrading bacteria at the soil–litter interface: comparing isolates with T-RFLP-based community profiles
FEMS Microbiol. Ecol. 86 (1), 45 - 58