Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1080/08958370802020810 |
| Titel (primär) | Chlorobenzene induces the NF-kB and p38 MAP kinase pathways in lung epithelial cells |
| Autor | Röder-Stolinski, C.; Fischäder, G.; Oostingh, G.J.; Eder, K.; Duschl, A.; Lehmann, I. |
| Quelle | Inhalation Toxicology |
| Erscheinungsjahr | 2008 |
| Department | IMMU |
| Band/Volume | 20 |
| Heft | 9 |
| Seite von | 813 |
| Seite bis | 820 |
| Sprache | englisch |
| Abstract | Chlorobenzene is a volatile organic compound that is used as a solvent in many industrial settings and has been shown to be related with irritations of the respiratory tract. Exposure to chlorobenzene induces the release of monocyte chemoattractant protein 1 (MCP-1) by lung epithelial cells, a chemokine involved in inflammatory reactions. To characterize the underlying mechanisms we investigated the influence of chlorobenzene on the activation of two intracellular signalling pathways: the nuclear factor-kappa B (NF-k B) and the p38 mitogen-activated protein kinase (MAPK) pathways. Human lung epithelial cells (A549) were stimulated with tumor necrosis factor (TNF)- in the presence or absence of specific inhibitors of NF-kB or the p38 MAP kinase and exposed to chlorobenzene using an air-liquid cell culture system. Exposure of lung epithelial cells to chlorobenzene resulted in an activation of NF-k B and p38 MAP kinase and a release of the chemokine MCP-1. In the presence of IKK-NBD, a specific NF-k B inhibitor, or the inhibitors of the p38 MAP kinase SB 203580 and SB 202190, the chlorobenzene-related MCP-1 release was suppressed, suggesting an involvement of both pathways in the chlorobenzene induced expression of MCP-1. Our data show that the release of MCP-1 following chlorobenzene exposure is dependent on the NF-k B and MAPK pathways. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=1376 |
| Röder-Stolinski, C., Fischäder, G., Oostingh, G.J., Eder, K., Duschl, A., Lehmann, I. (2008): Chlorobenzene induces the NF-kB and p38 MAP kinase pathways in lung epithelial cells Inhal. Toxicol. 20 (9), 813 - 820 10.1080/08958370802020810 |
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