Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1007/s00253-013-4867-0
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Titel (primär) Microbial community structure and dynamics during anaerobic digestion of various agricultural waste materials
Autor Ziganshin, A.M.; Liebetrau, J.; Pröter, J.; Kleinsteuber, S. ORCID logo
Quelle Applied Microbiology and Biotechnology
Erscheinungsjahr 2013
Department UMB
Band/Volume 97
Heft 11
Seite von 5161
Seite bis 5174
Sprache englisch
Keywords Biogas; Co-digestion; 16S rRNA genes; T-RFLP; Pyrosequencing
UFZ Querschnittsthemen RU4;
Abstract The influence of the feedstock type on the microbial communities involved in anaerobic digestion was investigated in laboratory-scale biogas reactors fed with different agricultural waste materials. Community composition and dynamics over 2 months of reactors’ operation were investigated by amplicon sequencing and profiling terminal restriction fragment length polymorphisms of 16S rRNA genes. Major bacterial taxa belonged to the Clostridia and Bacteroidetes, whereas the archaeal community was dominated by methanogenic archaea of the orders Methanomicrobiales and Methanosarcinales. Correlation analysis revealed that the community composition was mainly influenced by the feedstock type with the exception of a temperature shift from 38 to 55 °C which caused the most pronounced community shifts. Bacterial communities involved in the anaerobic digestion of conventional substrates such as maize silage combined with cattle manure were relatively stable and similar to each other. In contrast, special waste materials such as chicken manure or Jatropha press cake were digested by very distinct and less diverse communities, indicating partial ammonia inhibition or the influence of other inhibiting factors. Anaerobic digestion of chicken manure relied on syntrophic acetate oxidation as the dominant acetate-consuming process due to the inhibition of aceticlastic methanogenesis. Jatropha as substrate led to the enrichment of fiber-degrading specialists belonging to the genera Actinomyces and Fibrobacter.
dauerhafte UFZ-Verlinkung
Ziganshin, A.M., Liebetrau, J., Pröter, J., Kleinsteuber, S. (2013):
Microbial community structure and dynamics during anaerobic digestion of various agricultural waste materials
Appl. Microbiol. Biotechnol. 97 (11), 5161 - 5174 10.1007/s00253-013-4867-0