Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Analysis of structure, function, and activity of a benzene-degrading microbial community
Autor Jechalke, S.; Franchini, A.; Bastida, F.; Bombach, P.; Rosell, M.; Seifert, J.; von Bergen, M.; Vogt, C.; Richnow, H.H.;
Journal / Serie FEMS Microbiology Ecology
Erscheinungsjahr 2013
Department ISOBIO; UBT; PROTEOM;
Band/Volume 85
Heft 1
Sprache englisch;
POF III (gesamt) T41;
Keywords benzene degradation; compound-specific stable isotope analysis; protein-SIP/RNA-SIP; stable isotope probing
UFZ Querschnittsthemen ru3
Abstract We identified phylotypes performing distinct functions related to benzene degradation in complex microbial biofilms from an aerated treatment pond containing coconut textile. RNA- and protein-stable isotope probing (SIP) and compound-specific stable isotope analysis were applied to delineate bacteria and predominant pathways involved in the degradation of benzene. In laboratory microcosms, benzene was degraded at rates of ≥ 11 μM per day and per gram coconut textile under oxic conditions. Carbon isotope fractionation with isotopic enrichment factors (ε) of −0.6 to −1‰ and no significant hydrogen isotope fractionation indicated a dihydroxylation reaction for the initial ring attack. The incubation with [13C6]-benzene led to 13CO2 formation accompanied by 13C-labeling of RNA and proteins of the active biomass. Phylogenetic analysis of the 13C-labeled RNA revealed that phylotypes related to Zoogloea, Ferribacterium, Aquabacterium, and Hydrogenophaga within the Betaproteobacteria predominantly assimilated carbon from benzene. Although the phylogenetic classification of identified 13C-labeled proteins was biased by the incomplete metagenome information of public databases, it matched with RNA-SIP results at genus level. The detection of 13C-labeled proteins related to toluene dioxygenase and catechol 2,3-dioxygenase suggests benzene degradation by a dihydroxylation pathway with subsequent meta-cleavage of formed catechol.
ID 13519
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=13519
Jechalke, S., Franchini, A., Bastida, F., Bombach, P., Rosell, M., Seifert, J., von Bergen, M., Vogt, C., Richnow, H.H. (2013):
Analysis of structure, function, and activity of a benzene-degrading microbial community
FEMS Microbiol. Ecol. 85 (1), 14 - 26