Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Sulfated hyaluronan containing collagen matrices enhance cell-matrix-interaction, endocytosis, and osteogenic differentiation of human mesenchymal stromal cells
Autor Kliemt, S.; Lange, C.; Otto, W.; Hintze, V.; Möller, S.; von Bergen, M.; Hempel, U.; Kalkhof, S.;
Journal / Serie Journal of Proteome Research
Erscheinungsjahr 2013
Department PROTEOM;
Band/Volume 12
Heft 1
Sprache englisch;
POF III (gesamt) F11;
UFZ Querschnittsthemen ru3
Abstract

Inorganic-organic composite implant materials mimicking the environment of bone are promising applications to meet the increasing demands on biomaterials for bone regeneration caused by extended life spans and the concomitantly increase of bone treatments. Besides collagen type I (Col-l), glycosaminoglycans (GAG), such as hyaluronan, are important components of the bone extracellular matrix (ECM). Sulfated GAGs are potential stimulators of bone anabolic activity, as they are involved in the recruitment of mesenchymal stromal cells (MSCs) to the site of bone formation, and support differentiation to osteoblasts. Nevertheless, no consecutive data is currently available about the interaction of hyaluronan or sulfated hyaluronan-derivatives with hMSCs and the molecular processes being consequently regulated. We applied quantitative proteomics to investigate the influence of artificial ECM composed of Col-I and hyaluronan (Hya) or sulfated hyaluronan (HyaS3) on the molecular adaptation of osteogenic-differentiated hMSCs. Of the 1,370 quantified proteins, the expression of 4-11% was altered due to both aECM-combinations. Our results indicate that HyaS3 enhanced multiple cell functions, including cell-matrix-interaction, cell-signaling, endocytosis and differentiation. In conclusion, this study provides fundamental insights into regulative cellular responses associated with HyaS3 and Hya as components of aECM and underlines the potential of HyaS3 as a promising implant-coating-material.
ID 13114
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=13114
Kliemt, S., Lange, C., Otto, W., Hintze, V., Möller, S., von Bergen, M., Hempel, U., Kalkhof, S. (2013):
Sulfated hyaluronan containing collagen matrices enhance cell-matrix-interaction, endocytosis, and osteogenic differentiation of human mesenchymal stromal cells
J. Proteome Res. 12 (1), 378 - 389