||The LINA cohort: indoor chemical exposure, circulating eosinophil/basophil (Eo/B) progenitors and early life skin manifestations
||Weisse, K.; Lehmann, I.; Heroux, D.; Kohajda, T.; Herberth, G.
; Röder, S.
; von Bergen, M.; Borte, M.; Denburg, J.
|Journal / Serie
||Clinical and Experimental Allergy
||IMMU; PROTEOM; STUDIEN
||atopic dermatitis; cradle cap; eosinophil/basophil progenitors; indoor chemical exposure; LINA ; 1-year-old children; smoking; VOC
progenitor cells, especially those committed to the Eo/B lineage, are
known to contribute to allergic inflammation.
aim of the present study was to investigate whether environmental
factors are associated with changes in numbers of circulating Eo/B
progenitors at 1 year of age.
blood from 60 1-year-old children enrolled in the LINA (Lifestyle and
environmental factors and their Influence on Newborns Allergy risk)
birth cohort was assessed for Eo/B progenitor cells (Eo/B CFU) using
standardized and validated methylcellulose assays. Frozen peripheral
blood mononuclear cells (PBMC) were cultured in the presence of IL-3,
IL-5 or GM-CSF, and Eo/B CFUs enumerated. Clinical outcomes and exposure
to environmental tobacco smoke (ETS) were documented by standardized
questionnaires, and indoor volatile organic compound (VOC)
concentrations were assessed by passive sampling.
with skin manifestations (atopic dermatitis or cradle cap) within the
first year of life had higher numbers of circulating IL-3-, IL-5- or
GM-CSF-stimulated Eo/B CFUs (P < 0.05) at 1 year. In
children with cradle cap, a positive correlation was found between Eo/B
CFUs and exposure to ETS-related VOCs during pregnancy or at 1 year of
age (P < 0.05).
Conclusions and Clinical Relevance
is the first demonstration that environmental exposures are positively
associated with levels of circulating Eo/B progenitors. The recruitment
and differentiation of Eo/B progenitors in response to environmental
triggers may play a role in the development of skin manifestations
during the first year of life.
|Weisse, K., Lehmann, I., Heroux, D., Kohajda, T., Herberth, G., Röder, S., von Bergen, M., Borte, M., Denburg, J. (2012):
The LINA cohort: indoor chemical exposure, circulating eosinophil/basophil (Eo/B) progenitors and early life skin manifestations
Clin. Exp. Allergy 42 (9), 1337 - 1346