Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Comparative evaluation of particle properties, formation of reactive oxygen species and genotoxic potential of tungsten carbide based nanoparticles in vitro
Autor Kühnel, D.; Scheffler, K.; Wellner, P.; Meißner, T.; Potthoff, A.; Busch, W.; Springer, A.; Schirmer, K.;
Journal / Serie Journal of Hazardous Materials
Erscheinungsjahr 2012
Department BIOTOX;
Band/Volume 227-228
Sprache englisch;
Keywords micronucleus formation; reactive oxygen species (ROS); DNA damage; nanoparticle properties; characterization
Abstract

Tungsten carbide (WC) and cobalt (Co) are constituents of hard metals and are used for the production of extremely hard tools. Previous studies have identified greater cytotoxic potential of WC-based nanoparticles if particles contained Co. The aim of this study was to investigate whether the formation of reactive oxygen species (ROS) and micronuclei would help explain the impact on cultured mammalian cells by three different tungsten-based nanoparticles (WCS, WCL, WCL–Co (S: small; L: large)). The selection of particles allowed us to study the influence of particle properties, e.g. surface area, and the presence of Co on the toxicological results. WCS and WCL/WCL–Co differed in their crystalline structure and surface area, whereas WCS/WCL and WCL–Co differed in their cobalt content. WCL and WCL–Co showed neither a genotoxic potential nor ROS induction. Contrary to that, WCS nanoparticles induced the formation of both ROS and micronuclei. CoCl2 was tested in relevant concentrations and induced no ROS formation, but increased the rate of micronuclei at concentrations exceeding those present in WCL–Co. In conclusion, ROS and micronuclei formation could not be associated with the presence of Co in the WC-based particles. The contrasting responses elicited by WCS vs. WCL appear to be due to large differences in crystalline structure.


ID 12564
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=12564
Kühnel, D., Scheffler, K., Wellner, P., Meißner, T., Potthoff, A., Busch, W., Springer, A., Schirmer, K. (2012):
Comparative evaluation of particle properties, formation of reactive oxygen species and genotoxic potential of tungsten carbide based nanoparticles in vitro
J. Hazard. Mater. 227-228 , 418 - 426