Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) MALDI-typing of infectious algae of the genus Prototheca using SOM portraits
Autor Wirth, H.; von Bergen, M.; Murugaiyan, J.; Roesler, U.; Stokowy, T.; Binder, H.;
Journal / Serie Journal of Microbiological Methods
Erscheinungsjahr 2012
Department PROTEOM;
Band/Volume 88
Heft 1
Sprache englisch;
Keywords MALDI-typing; Mass spectrometry; Peaklist; Prototheca; SOM; Classification
Abstract

Background

MALDI-typing has become a frequently used approach for the identification of microorganisms and recently also of invertebrates. Similarity-comparisons are usually based on single-spectral data. We apply self-organizing maps (SOM) to portray the MS-spectral data with individual resolution and to improve the typing of Prototheca algae by using meta-spectra representing prototypes of groups of similar-behaving single spectra.

Results

The MALDI-TOF peaklists of more than 300 algae extracts referring to five Prototheca species were transformed into colored mosaic images serving as molecular portraits of the individual samples. The portraits visualize the algae-specific distribution of high- and low-amplitude peaks in two dimensions. Species-specific pattern of MS intensities were readily discernable in terms of unique single spots of high amplitude MS-peaks which collect characteristic fingerprint spectra. The spot patterns allow the visual identification of groups of samples referring to different species, genotypes or isolates. The use of meta-peaks instead of single-peaks reduces the dimension of the data and leads to an increased discriminating power in downstream analysis.

Conclusions

We expect that our SOM portray method improves MS-based classifications and feature selection in upcoming applications of MALDI-typing based species identifications especially of closely related species.

ID 12153
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=12153
Wirth, H., von Bergen, M., Murugaiyan, J., Roesler, U., Stokowy, T., Binder, H. (2012):
MALDI-typing of infectious algae of the genus Prototheca using SOM portraits
J. Microbiol. Methods 88 (1), 83 - 89