Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.marenvres.2008.02.027
Titel (primär) Teasing apart activities of different types of ABC efflux pumps in bivalve gills using the concepts of independent action and concentration addition
Autor Luckenbach, T. ORCID logo ; Altenburger, R.; Epel, D.
Quelle Marine Environmental Research
Erscheinungsjahr 2008
Department BIOTOX; ZELLTOX
Band/Volume 66
Heft 1
Seite von 75
Seite bis 76
Sprache englisch
Keywords Concentration addition; Independent action; Mixtures; MRP; Multixenobiotic resistance; Mytilus californianus; P-gp; concentration addition; independent action; mixtures; multixenobiotic resistance
Abstract Fluorescent dyes and inhibitor compounds are commonly used to detect activity of multixenobiotic resistance (MXR) efflux pumps in marine invertebrates. We here address the question whether compounds acting as specific inhibitors of certain mammalian transporters can be used in dye efflux assays to distinguish different transporter activities in gill tissue from a marine mussel. We quantified effects of PSC833, a specific inhibitor of mammalian P-gp (P-glycoprotein, ABCB1), and MK571, which blocks MRP (Multidrug resistance associated protein, ABCC) type transporters, on calcein-am efflux in gill tissue of Mytilus californianus. Calcein-am acts as a substrate of both P-gp and MRP. Effects of single compounds and mixtures were determined and combined effect models predicting independent action (IA) and concentration addition (CA) of the chemicals were applied. Effect values predicted by IA showed better correspondence with the experimentally obtained data. This indicates that the inhibitor compounds target different mechanisms of calcein-am efflux and points to P-gp and MRP activities in mussel gills. Our approach could be a simple way for identifying the efflux transporter types targeted by chemosensitizers, including environmentally relevant compounds, in native tissues from marine invertebrates.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=1197
Luckenbach, T., Altenburger, R., Epel, D. (2008):
Teasing apart activities of different types of ABC efflux pumps in bivalve gills using the concepts of independent action and concentration addition
Mar. Environ. Res. 66 (1), 75 - 76 10.1016/j.marenvres.2008.02.027