Details zur Publikation
Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.soilbio.2011.10.017
Titel (primär) Feasibility of a cell separation-proteomic based method for soils with different edaphic properties and microbial biomass
Autor Bastida, F.; Algora, C.; Hernández, T.; García, C.
Quelle Soil Biology & Biochemistry
Erscheinungsjahr 2012
Department ISOBIO
Band/Volume 45
Seite von 136
Seite bis 138
Sprache englisch
Keywords Soil proteomics; Fluorescence microscopy; Cell extraction; Texture; Salinity; Organic carbon
Abstract

The suitability of a soil proteome analysis based on previous cell extraction by gradient centrifugation was tested in semiarid soils with distinct edaphic properties and microbial biomass after enrichment with carbon and nitrogen. A sandy loam soil with low organic carbon content reached higher microbial biomass (estimated by PLFAs) after stimulation with nutrient sources (glucose and proline) than a naturally rich soil. However, the extractability of soil microbial cells was higher in a poor soil with high electrical conductivity probably due to the high saline content. The number of identified proteins in the poor soils reached 71 with proteins related to energy processes, transport and nucleic acid metabolism representing the highest percentage. High organic carbon content negatively influences cell extraction and protein separation and analysis. Soil texture and/or salinity might be related to the expression of proteins involved in the removal of reactive oxygen species (ROS) such catalase and superoxide dismutase (SOD) under active metabolism and microbial biomass development
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11925
Bastida, F., Algora, C., Hernández, T., García, C. (2012):
Feasibility of a cell separation-proteomic based method for soils with different edaphic properties and microbial biomass
Soil Biol. Biochem. 45 , 136 - 138 10.1016/j.soilbio.2011.10.017