Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.mimet.2008.04.004
Titel (primär) Miniaturized calometry - a new method for real-time biofilm activity analysis
Autor Lerchner, J.; Wolf, A.; Buchholz, F.; Mertens, F.; Neu, T.R.; Harms, H.; Maskow, T. ORCID logo
Quelle Journal of Microbiological Methods
Erscheinungsjahr 2008
Department UMB; FLOEK
Band/Volume 74
Heft 2-3
Seite von 74
Seite bis 81
Sprache englisch
Keywords Biofilm activity; Biofilm modeling; Chip calorimeter; Biothermodynamics; Pseudomonas putida
Abstract The partial dissipation of Gibbs energy as heat reflects the metabolic dynamic of biofilms in real time and may also allow quantitative conclusions about the chemical composition of the biofilm via Hess' law. Presently, the potential information content of heat is hardly exploited due to the low flexibility, the low throughput and the high price of conventional calorimeters. In order to overcome the limitations of conventional calorimetry a miniaturized calorimeter for biofilm investigations has been evaluated. Using four thermopiles a heat production with spatial and temporal resolutions of 2.5 cm- 1 and 2 s- 1 could be determined. The limit of detection of the heat flow measurement was 20 nW, which corresponds to the cell density of an early stage biofilm (approx. 3 × 105 cells cm- 2). By separating biofilm cultivation from the actual heat measurement, a high flexibility and a much higher throughput was achieved if compared with conventional calorimeters. The approach suggested allows cultivation of biofilms in places of interest such as technological settings as well as in nature followed by highly efficient measurements in the laboratory. Functionality of the miniaturized calorimeter was supported by parallel measurements with confocal laser scanning microscopy and a fiber optic based oxygen sensor using the oxycaloric equivalent (- 460 kJ mol-O2- 1).
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=1185
Lerchner, J., Wolf, A., Buchholz, F., Mertens, F., Neu, T.R., Harms, H., Maskow, T. (2008):
Miniaturized calometry - a new method for real-time biofilm activity analysis
J. Microbiol. Methods 74 (2-3), 74 - 81 10.1016/j.mimet.2008.04.004