Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1021/es2026196
Titel (primär) A bench-scale constructed wetland as a model to characterize benzene biodegradation processes in freshwater wetlands
Autor Rakoczy, J.; Remy, B.; Vogt, C.; Richnow, H.H.
Quelle Environmental Science & Technology
Erscheinungsjahr 2011
Department ISOBIO
Band/Volume 45
Heft 23
Seite von 10036
Seite bis 10044
Sprache englisch
Abstract
In wetlands, a variety of biotic and abiotic processes can contribute to the removal of organic substances. Here, we used compound-specific isotope analysis (CSIA), hydrogeochemical parameters and detection of functional genes to characterize in situ biodegradation of benzene in a model constructed wetland over a period of 370 days. Despite low dissolved oxygen concentrations (<30 μM), the oxidation of ammonium to nitrate and the complete oxidation of ferrous iron pointed to a dominance of aerobic processes, suggesting efficient oxygen transfer into the sediment zone by plants. As benzene removal became highly efficient after day 231 (>98% removal), we applied CSIA to study in situ benzene degradation by indigenous microbes. Combining carbon and hydrogen isotope signatures by two-dimensional stable isotope analysis revealed that benzene was degraded aerobically, mainly via the monohydroxylation pathway. This was additionally supported by the detection of the BTEX monooxygenase gene tmoA in sediment and root samples. Calculating the extent of biodegradation from the isotope signatures demonstrated that at least 85% of benzene was degraded by this pathway and thus, only a small fraction was removed abiotically. This study shows that model wetlands can contribute to an understanding of biodegradation processes in floodplains or natural wetland systems.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11736
Rakoczy, J., Remy, B., Vogt, C., Richnow, H.H. (2011):
A bench-scale constructed wetland as a model to characterize benzene biodegradation processes in freshwater wetlands
Environ. Sci. Technol. 45 (23), 10036 - 10044 10.1021/es2026196