Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Degradation of the antibiotics norfloxacin and ciprofloxacin by a white-rot fungus and identification of degradation products
Autor Prieto, A.; Möder, M.; Rodil, R.; Adrian, L.; Marco-Urrea, E.;
Journal / Serie Bioresource Technology
Erscheinungsjahr 2011
Department ISOBIO; ANA;
Band/Volume 102
Heft 23
Sprache englisch;
Keywords Fluoroquinolones; Trametes versicolor; Pharmaceuticals; Biodegradation; Laccase
Abstract

More than 90% of the antibiotics ciprofloxacin (CIPRO) and norfloxacin (NOR) at 2 mg L−1 were degraded by Trametes versicolor after 7 days of incubation in malt extract liquid medium. In in vitro assays with purified laccase (16.7 nkat mL−1), an extracellular enzyme excreted constitutively by this fungus, 16% of CIPRO was removed after 20 h. The addition of the laccase mediator 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt led to 97.7% and 33.7% degradation of CIPRO and NOR, respectively. Inhibition of CIPRO and NOR degradation by the cytochrome P450 inhibitor 1-aminobenzotriazole suggests that the P450 system also plays a role in the degradation of the two antibiotics. Transformation products of CIPRO and NOR were monitored at different incubation times by triple-quadrupole and quadrupole time-of-flight mass spectrometry, and can be assigned to three different reaction pathways: (i) oxidation of the piperazinyl substituent, (ii) monohydroxylation, and (iii) formation of dimeric products.
ID 11685
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11685
Prieto, A., Möder, M., Rodil, R., Adrian, L., Marco-Urrea, E. (2011):
Degradation of the antibiotics norfloxacin and ciprofloxacin by a white-rot fungus and identification of degradation products
Bioresour. Technol. 102 (23), 10987 - 10995