Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1007/s00253-011-3497-7 |
| Volltext | Shareable Link |
| Titel (primär) | Potentials and limitations of miniaturized calorimeters for bioprocess monitoring |
| Autor | Maskow, T.
; Schubert, T.; Wolf, A.; Buchholz, F.; Regestein, L.; Buechs, J.; Mertens, F.; Harms, H.; Lerchner, J. |
| Quelle | Applied Microbiology and Biotechnology |
| Erscheinungsjahr | 2011 |
| Department | UMB; UV |
| Band/Volume | 92 |
| Heft | 1 |
| Seite von | 55 |
| Seite bis | 66 |
| Sprache | englisch |
| Keywords | Calorimetry; Biothermodynamics; Bioprocess control; Biofilms; Chip calorimetry; FIA; At-line |
| Abstract | In theory, heat production rates are very well suited for analysing and
controlling bioprocesses on different scales from
a few nanolitres up to many cubic metres. Any bioconversion
is accompanied by a production (exothermic) or consumption (endothermic)
of heat. The heat is tightly connected with the
stoichiometry of the bioprocess via the law of Hess, and its rate is
connected
to the kinetics of the process. Heat signals provide
real-time information of bioprocesses. The combination of heat
measurements
with respirometry is theoretically suited for the
quantification of the coupling between catabolic and anabolic reactions.
Heat measurements have also practical advantages. Unlike
most other biochemical sensors, thermal transducers can be mounted
in a protected way that prevents fouling, thereby minimizing
response drifts. Finally, calorimetry works in optically opaque
solutions and does not require labelling or reactants. It is
surprising to see that despite all these advantages, calorimetry
has rarely been applied to monitor and control bioprocesses
with intact cells in the laboratory, industrial bioreactors or
ecosystems. This review article analyses the reasons for
this omission, discusses the additional information calorimetry can
provide in comparison with respirometry and presents
miniaturization as a potential way to overcome some inherent weaknesses
of conventional calorimetry. It will be discussed for which
sample types and scientific question miniaturized calorimeter
can be advantageously applied. A few examples from different
fields of microbiological and biotechnological research will
illustrate the potentials and limitations of chip
calorimetry. Finally, the future of chip calorimetry is addressed in an
outlook. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11642 |
| Maskow, T., Schubert, T., Wolf, A., Buchholz, F., Regestein, L., Buechs, J., Mertens, F., Harms, H., Lerchner, J. (2011): Potentials and limitations of miniaturized calorimeters for bioprocess monitoring Appl. Microbiol. Biotechnol. 92 (1), 55 - 66 10.1007/s00253-011-3497-7 |
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