Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) The aromatic volatile organic compounds toluene, benzene and styrene induce COX-2 and prostaglandins in human lung epithelial cells via oxidative stress and p38 MAPK activation
Autor Mögel, I.; Baumann, S.; Böhme, A.; Kohajda, T.; von Bergen, M.; Simon, J.-C.; Lehmann, I.;
Journal / Serie Toxicology
Erscheinungsjahr 2011
Department OEC; IMMU; METABOX; PROTEOM;
Band/Volume 289
Heft 1
Sprache englisch;
Keywords Volatile organic compound (VOC); Lung epithelial cells; Cyclooxygenase-2 (COX-2); Oxidative stress; p38 MAPK
Abstract
Toluene, benzene and styrene are volatile organic compounds (VOCs) widely distributed in the environment. Tobacco smoke, traffic exposure and solvents used for paints, rubber and adhesives are known sources for these compounds. The aim of the present study was to investigate whether toluene, benzene and styrene can induce inflammatory reactions in lung cells and to characterize possible underlying mechanisms. A previous study gave evidence that expression of cyclooxygenase-2 (COX-2) is upregulated following exposure to the aromatic VOC chlorobenzene. Here, we investigated the effects of the aromatics toluene, benzene and styrene on human lung cells, with emphasis on COX-2, the rate-limiting enzyme of the prostaglandin pathway. In addition, we studied the potential role of oxidative stress and p38 MAPK activation in the toluene/benzene/styrene-dependent COX-2 induction. Following exposure to the aromatic compounds the expression level of COX-2 increased markedly. In addition, prostaglandin E2 (PGE2) and prostaglandin F (PGF), major products of the COX enzyme, were found to be upregulated in response to toluene, benzene or styrene exposure. Furthermore, we observed an activation of p38 MAPK resulting from aromatic VOC exposure. Treatment of the cells with a specific p38 inhibitor (SB203580) or the antioxidant N-acetylcysteine (NAC) was able to prevent the toluene/benzene/styrene-dependent COX-2 activation, and subsequent increased PGE2 and PGF secretion. These results suggest that toluene, benzene and styrene induce production and secretion of PGE2 and PGF in lung epithelial cells via p38 MAPK and COX-2 activation in a redox sensitive manner.
ID 11474
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11474
Mögel, I., Baumann, S., Böhme, A., Kohajda, T., von Bergen, M., Simon, J.-C., Lehmann, I. (2011):
The aromatic volatile organic compounds toluene, benzene and styrene induce COX-2 and prostaglandins in human lung epithelial cells via oxidative stress and p38 MAPK activation
Toxicology 289 (1), 28 - 37