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Titel (primär) Microbial populations related to PAH biodegradation in an aged biostimulated creosote-contaminated soil
Autor Llado, S.; Jiménez, N.; Viñas, M.; Solanas, A.M.;
Journal / Serie Biodegradation
Erscheinungsjahr 2009
Department ISOBIO;
Band/Volume 20
Heft 5
Sprache englisch;
Keywords Polycyclic aromatic hydrocarbons; Biodegradation; Bioavailability; Bioremediation; Pyrene; Chrysene; 16SrRNA; DGGE
Abstract

A previous bioremediation survey on a creosote-contaminated soil showed that aeration and optimal humidity promoted depletion of three-ringed polycyclic aromatic hydrocarbons (PAHs), but residual concentrations of four-ringed benzo(a)anthracene (B(a)A) and chrysene (Chry) remained. In order to explain the lack of further degradation of heavier PAHs such as four-ringed PAHs and to analyze the microbial population responsible for PAH biodegradation, a chemical and microbial molecular approach was used. Using a slurry incubation strategy, soil in liquid mineral medium with and without additional B(a)A and Chry was found to contain a powerful PAH-degrading microbial community that eliminated 89% and 53% of the added B(a)A and Chry, respectively. It is hypothesized that the lack of PAH bioavailability hampered their further biodegradation in the unspiked soil. According to the results of the culture-dependent and independent techniques Mycobacterium parmense, Pseudomonas mexicana, and Sphingobacterials group could control B(a)A and Chry degradation in combination with several microorganisms with secondary metabolic activity.

ID 11323
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11323
Llado, S., Jiménez, N., Viñas, M., Solanas, A.M. (2009):
Microbial populations related to PAH biodegradation in an aged biostimulated creosote-contaminated soil
Biodegradation 20 (5), 593 - 601