Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Assimilation of benzene carbon through multiple trophic levels traced by different stable isotope probing methodologies
Autor Bastida, F.; Jechalke, S.; Bombach, P.; Franchini, A.G.; Seifert, J.; von Bergen, M.; Vogt, C.; Richnow, H.H.;
Journal / Serie FEMS Microbiology Ecology
Erscheinungsjahr 2011
Department ISOBIO; UBT; PROTEOM;
Band/Volume 77
Heft 2
Sprache englisch;
Keywords stable isotope probing;fatty acid-SIP;protein-SIP;amino acid-SIP;benzene degradation;food web
Abstract

The flow of benzene carbon along a food chain consisting of bacteria and eukaryotes, including larvae (Diptera: Chironomidae), was evaluated by total lipid fatty acids (TLFAs)-, amino acid- and protein-stable isotope probing (SIP). A coconut-fibre textile, colonized by a benzene-degrading biofilm, was sampled in a system established for the remediation of benzene, toluene, ethylbenzene and xylenes (BTEX)-polluted groundwater and incubated with 12C- and [13C6]-benzene (>99 at.%) in a batch-scale experiment for 2–8 days. After 8 days, Chironomus sp. larvae were added to study carbon flow to higher trophic levels. Gas chromatography-combustion-isotope ratio monitoring mass spectrometry of TLFA showed increased isotope ratios in the 13C-benzene-incubated biofilm. A higher 13C-enrichment was observed in TLFAs, indicative of Gram-negative bacteria than for Gram-positive. Fatty acid indicators of eukaryotes showed significant 13C-incorporation, but to a lower extent than bacterial indicators. Fatty acids extracted from larvae feeding on 13C-biofilm reached an isotopic ratio of 1.55 at.%, illustrating that the larvae feed, to some extent, on labelled biomass. No 13C-incorporation was detectable in larval proteins after their separation by sodium-dodecyl sulphate-polyacrylamide gel electrophoresis and analysis by nano-liquid-chromatography-mass spectrometry. The flow of benzene-derived carbon could be traced in a food web consisting of bacteria and eukaryotes.

ID 11150
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11150
Bastida, F., Jechalke, S., Bombach, P., Franchini, A.G., Seifert, J., von Bergen, M., Vogt, C., Richnow, H.H. (2011):
Assimilation of benzene carbon through multiple trophic levels traced by different stable isotope probing methodologies
FEMS Microbiol. Ecol. 77 (2), 357 - 369