Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Pseudechis australis venomics: adaptation for a defense against microbial pathogens and recruitment of body transferrin
Autor Georgieva, D.; Seifert, J.; Öhler, M.; von Bergen, M.; Spencer, P.; Arni, R.K.; Genov, N.; Betzel, C.;
Journal / Serie Journal of Proteome Research
Erscheinungsjahr 2011
Department PROTEOM;
Band/Volume 10
Heft 5
Sprache englisch;
Keywords snake venomics; Pseudechis australis; 2-D electrophoresis; electrospray mass spectrometry; venom transferrin; enzyme activity
Abstract

The venom composition of Pseudechis australis, a widely distributed in Australia reptile, was analyzed by 2-DE and mass spectrometric analysis. In total, 102 protein spots were identified as venom toxins. The gel is dominated by horizontal trains of spots with identical or very similar molecular masses but differing in the pI values. This suggests possible post-translational modifications of toxins, changing their electrostatic charge. The results demonstrate a highly specialized biosynthesis of toxins destroying the hemostasis (P-III metalloproteases, SVMPs), antimicrobial proteins (l-amino acid oxidases, LAAOs, and transferrin-like proteins, TFLPs), and myotoxins (phospholipase A2s, PLA2s). The three transferrin isoforms of the Australian P. australis (Elapidae snake) venom are highly homologous to the body transferrin of the African Lamprophis fuliginosus (Colubridae), an indication for the recruitment of body transferrin. The venomic composition suggests an adaptation for a defense against microbial pathogens from the prey. Transferrins have not previously been reported as components of elapid or other snake venoms. Ecto-5'-nucleotidases (5'-NTDs), nerve growth factors (VNGFs), and a serine proteinase inhibitor (SPI) were also identified. The venom composition and enzymatic activities explain the clinical manifestation of the king brown snakebite. The results can be used for medical, scientific, and biotechnological purposes.

ID 11049
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11049
Georgieva, D., Seifert, J., Öhler, M., von Bergen, M., Spencer, P., Arni, R.K., Genov, N., Betzel, C. (2011):
Pseudechis australis venomics: adaptation for a defense against microbial pathogens and recruitment of body transferrin
J. Proteome Res. 10 (5), 2440 - 2464