Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1111/j.1574-6976.2011.00266.x
Titel (primär) Fungi in freshwaters: ecology, physiology and biochemical potential
Autor Krauss, G.-J.; Solé, M.; Krauss, G.; Schlosser, D. ORCID logo ; Wesenberg, D.; Bärlocher, F.
Quelle FEMS Microbiology Reviews
Erscheinungsjahr 2011
Department UMB
Band/Volume 35
Heft 4
Seite von 620
Seite bis 651
Sprache englisch
Keywords aquatic fungi; ecosystem functions; laccase; sulfate; glutathione; phytochelatin; metallothionein
Abstract The high cost of commercial lipases limits their industrial application in the production of biodiesel or fatty acid methyl esters (FAME). This disadvantage has encouraged the search for lipase-producing microorganisms (LPMs) as potential whole cell catalysts for FAME production. The aim of this study, therefore, was to evaluate innovative procedures for easy selection and testing of LPMs as a low-cost whole cell catalyst, based on catalytic performance, methanol tolerance and physico-chemical cell surface properties. The latter (in particular the cell surface hydrophobicity and charge) were analyzed because of their crucial role in microbial adhesion to surfaces and the concomitant increase in cell immobilization and bioavailability of hydrophobic substrates. Biocatalysis experiments performed in the presence of nutrient, rapeseed oil and methanol were an effective tool for studying and identifying, in just two experiments, the capacity of different LPMs as biocatalysts in organic media, as well as the methanol tolerance of the cell and the lipase. This indicates the potential for using live microorganisms for FAME production. Another finding was that the inhibitory effect of methanol is more significant for lipase activity than LPM growth, indicating that the way in which alcohol is supplied to the reaction is a crucial step in FAME production by biocatalysts. According to these results, the application of these innovative assessments should simplify the search for new strains which are able to effectively catalyze the FAME production process.
dauerhafte UFZ-Verlinkung
Krauss, G.-J., Solé, M., Krauss, G., Schlosser, D., Wesenberg, D., Bärlocher, F. (2011):
Fungi in freshwaters: ecology, physiology and biochemical potential
FEMS Microbiol. Rev. 35 (4), 620 - 651 10.1111/j.1574-6976.2011.00266.x