Details zur Publikation |
Kategorie | Textpublikation |
Referenztyp | Zeitschriften |
DOI | 10.1016/j.tox.2011.03.012 |
Titel (primär) | T-2 toxin is a cytochrome P450 1A1 inducer and leads to MAPK/p38- but not aryl hydrocarbon receptor-dependent interleukin-8 secretion in the human intestinal epithelial cell line Caco-2 |
Autor | Kruber, P.; Trump, S.; Behrens, J.; Lehmann, I. |
Quelle | Toxicology |
Erscheinungsjahr | 2011 |
Department | IMMU |
Band/Volume | 284 |
Heft | 1-3 |
Seite von | 34 |
Seite bis | 41 |
Sprache | englisch |
Keywords | T-2 toxin; p38 MAPK; Aryl hydrocarbon receptor; CYP1A1; IL-8; Caco-2 |
Abstract |
T-2 toxin (T-2) is a secondary metabolite produced by various mould species of the genus Fusarium and a common contaminant detectable in staple foods of cereal origin. In the present study the impact of this mycotoxin on the inflammatory response of the intestinal epithelial cell line Caco-2 was examined by measuring interleukin (IL)-8 secretion. A T-2 concentration dependent IL-8 up-regulation was detected in IL-1ß stimulated and unstimulated Caco-2 cells. To elucidate the possible underlying molecular mechanism of this conditional T-2-provoked IL-8 induction, a possible involvement of the aryl hydrocarbon receptor (AHR) and the mitogen-activated protein kinase (MAPK) pathway was investigated. Like benzo-[a]-pyrene (B[a]P), a well known AHR ligand, T-2 led to cytochrome P450 1A1 (CYP1A1) mRNA expression in Caco-2 cells, which could be inhibited by the AHR antagonist resveratrol. However, resveratrol did not influence T-2-dependent IL-8 induction. Since T-2 did not lead to AHR-translocation in stably GFP-AHR-transfected cells, an AHR dependency of T-2-triggered IL-8 induction could be excluded. But finally, up to a total inhibition of T-2-induced IL-8 was obtained using p38 inhibitors. Therefore, we conclude that p38 MAPK is responsible for mediating the inflammatory properties of the type A trichothecene T-2. |
dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10978 |
Kruber, P., Trump, S., Behrens, J., Lehmann, I. (2011): T-2 toxin is a cytochrome P450 1A1 inducer and leads to MAPK/p38- but not aryl hydrocarbon receptor-dependent interleukin-8 secretion in the human intestinal epithelial cell line Caco-2 Toxicology 284 (1-3), 34 - 41 10.1016/j.tox.2011.03.012 |