Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1099/mic.0.044040-0
Titel (primär) KlHsl1 is a component of glycerol response pathways in the milk yeast Kluyveromyces lactis
Autor Cialfi, S.; Uccelletti, D.; Carducci, A.; Wésolowski-Louvel, M.; Mancini, P.; Heipieper, H.J. ORCID logo ; Saliola, M.
Quelle Microbiology-SGM
Erscheinungsjahr 2011
Department UBT
Band/Volume 157
Heft 5
Seite von 1509
Seite bis 1518
Sprache englisch
Keywords AA, antimycin A; CWI, cell wall integrity; FACS, fluorescence-activated cell sorting; MAPK, mitogen-activated protein kinase

In Saccharomyces cerevisiae, HSL1 (NIK1) encodes a serine-threonine protein kinase involved in cell cycle control and morphogenesis. Deletion of its putative orthologue in Kluyveromyces lactis, KlHSL1, gives rise to sensitivity to the respiratory inhibitor antimycin A (AA). Resistance to AA on glucose (Rag+ phenotype) is associated with genes (RAG) required for glucose metabolism/glycolysis. To understand the relationship between RAG and KlHSL1, rag and Klhsl1 mutant strains were investigated. The analysis showed that all the mutants contained a phosphorylated form of Hog1 and displayed an inability to synthesize/accumulate glycerol as a compatible solute. In addition, rag mutants also showed alterations in both cell wall and membrane fatty acids. The pleiotropic defects of these strains indicate that a common pathway regulates glucose utilization and stress response mechanisms, suggesting impaired adaptation of the plasma membrane/cell wall during the respiratory–fermentative transition. KlHsl1 could be the link between these adaptive pathways and the morphogenetic checkpoint.

dauerhafte UFZ-Verlinkung
Cialfi, S., Uccelletti, D., Carducci, A., Wésolowski-Louvel, M., Mancini, P., Heipieper, H.J., Saliola, M. (2011):
KlHsl1 is a component of glycerol response pathways in the milk yeast Kluyveromyces lactis
Microbiology-(UK) 157 (5), 1509 - 1518 10.1099/mic.0.044040-0