Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Repeated fed-batch fermentation using biosensor online control for citric acid production by Yarrowia lipolytica
Autor Moeller, L.; Grünberg, M.; Zehnsdorf, A.; Aurich, A.; Bley, T.; Strehlitz, B.;
Journal / Serie Journal of Biotechnology
Erscheinungsjahr 2011
Department UBZ;
Band/Volume 153
Heft 3-4
Sprache englisch;
Keywords citric acid; Yarrowia lipolytica; glucose biosensor; biosensor online control; semicontinuous fermentation; repeated fed-batch
Abstract Biosensor-controlled substrate feeding was used in a citric acid production process with the yeast strain Yarrowia lipolytica H222 with glucose as the carbon source. The application of an online glucose biosensor measurement facilitated the performance of long-time repeated fed-batch process with automated bioprocess control. Ten cycles of repeated fed-batch fermentation were carried out in order to validate both the stability of the microorganism for citric acid production and the robustness of the glucose biosensor in a long-time experiment. In the course of this fermentation with a duration of 553 h, a slight loss of productivity from 1.4 g/(L*h) to 1.1 g/(L*h) and of selectivity for citric acid from 91 % to 88 % was observed. The glucose biosensor provided 6,227 measurements without any loss of activity.
ID 10931
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10931
Moeller, L., Grünberg, M., Zehnsdorf, A., Aurich, A., Bley, T., Strehlitz, B. (2011):
Repeated fed-batch fermentation using biosensor online control for citric acid production by Yarrowia lipolytica
J. Biotechnol. 153 (3-4), 133 - 137