Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1007/s00216-010-4413-1 |
| Volltext | Shareable Link |
| Titel (primär) | A new approach for calibration of laser ablation inductively coupled plasma mass spectrometry using thin layers of spiked agarose gels as references |
| Autor | Stärk, H.-J.; Wennrich, R. |
| Quelle | Analytical and Bioanalytical Chemistry |
| Erscheinungsjahr | 2011 |
| Department | ANA |
| Band/Volume | 399 |
| Heft | 6 |
| Seite von | 2211 |
| Seite bis | 2217 |
| Sprache | englisch |
| Keywords | Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS); Calibration; Agarose thin-film references; Total consumption laser ablation; Trace element detection |
| Abstract | Calibration of analytical methods using laser ablation for sample introduction is often problematic. The availability of matrix-adapted standard materials is a crucial factor in the analysis of biological samples in particular. In this work a method for preparation of thin-film references for LA-ICP-MS is presented which is inexpensive, relatively simple and generally practicable. Aqueous solutions of agarose spiked with defined amounts of the analytes were cast on a carrier and then dried. When the thin-film references were characterized the average thickness of the films was 0.03 mm in the centre of the film and the relative standard deviation was 8%. Nebulization ICP-MS analysis after acid digestion of the agarose film was used to investigate the effectiveness of the spiking procedure. Recovery of the spiked elements was frequently in the range 90-110% (for rare earth elements 97-102%). Laser ablation ICP-MS analysis was used to investigate the distribution of the spiked elements in the film. When the laser was scanned across the gel the measured intensities were not constant, but had a peak-shaped profile with a flat top. Use of this flat-top region for analytical purposes, after its characterization by laser ablation ICP-MS, is proposed. Analysis of cell cultures was carried out by direct laser ablation-ICP-MS with the calibration method described. The results were in accordance with values previously achieved by nebulization ICP-MS. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10856 |
| Stärk, H.-J., Wennrich, R. (2011): A new approach for calibration of laser ablation inductively coupled plasma mass spectrometry using thin layers of spiked agarose gels as references Anal. Bioanal. Chem. 399 (6), 2211 - 2217 10.1007/s00216-010-4413-1 |
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