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Titel (primär) Chances and pitfalls of chemical cross-linking with amine-reactive N-hydroxysuccinimide esters
Autor Kalkhof, S.; Sinz, A.;
Journal / Serie Analytical and Bioanalytical Chemistry
Erscheinungsjahr 2008
Department PROTEOM;
Band/Volume 392
Heft 1-2
Sprache englisch;
Supplements https://static-content.springer.com/esm/art%3A10.1007%2Fs00216-008-2231-5/MediaObjects/216_2008_2231_MOESM1_ESM.pdf
Keywords Chemical cross-linking; Amine-reactive cross-linkers; Mass spectrometry
Abstract In this report we summarize our experiences with the reaction products of N-hydroxysuccinimide (NHS) esters, which are widely used for chemical cross-linking of lysine residues in proteins. We describe the products, which should be scrutinized during data analysis using customized software when NHS esters are employed for chemical cross-linking. Reaction products of NHS esters were observed not only with lysines, but also with serines, tyrosines, and threonines. This report is intended to be a practical guide for those working in the field of chemical cross-linking and mass spectrometry.



ID 1083
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=1083
Kalkhof, S., Sinz, A. (2008):
Chances and pitfalls of chemical cross-linking with amine-reactive N-hydroxysuccinimide esters
Anal. Bioanal. Chem. 392 (1-2), 305 - 312