Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) DIGE-based protein expression analysis of B[a]P-exposed hepatoma cells reveals a complex stress response including alterations in oxidative stress, cell cycle control, and cytoskeleton motility at toxic and subacute concentrations
Autor Dautel, F.; Kalkhof, S.; Trump, S.; Michaelson, J.; Beyer, A.; Lehmann, I.; von Bergen, M.;
Journal / Serie Journal of Proteome Research
Erscheinungsjahr 2011
Department IMMU; PROTEOM;
Band/Volume 10
Heft 2
Sprache englisch;
Keywords benzo[a]pyrene (B[a]P); aryl hydrocarbon receptor (Ahr); oxidative stress; human protein reference database (HPRD); protein expression analysis; DIGE
Abstract Although the effects of high concentrations of the carcinogen benzo[a]pyrene (B[a]P) have been studied extensively, little is known about its effects at subacute toxic concentrations, which are typical for environmental pollutants. We exposed murine Hepa1c1c7 cells to a toxic concentration (5 µM) and a subacute concentration (50 nM) of B[a]P over a period of 2-24 h to differentiate between acute and pseudochronic effects and conducted a time-course analysis of B[a]P-influenced protein expression by DIGE. In total, a set of 120 spots were found to be significantly altered due to B[a]P exposure of which 112 were subsequently identified by mass spectrometry. Clustering and principal component analysis were conducted to identify sets of proteins responding in a concerted manner to the exposure. Our results indicate an immediate response to the contaminant at the protein level and demonstrate that B[a]P exposure alters the cellular response by disturbing proteins involved in oxidative stress, cell cycle regulation, apoptosis, and cytoskeleton organization. Furthermore, network analysis of protein-protein interactions revealed a complex network of interacting, B[a]P-regulated proteins mostly belonging to the cytoskeleton organization and several signal transduction pathways.
ID 10734
dauerhafte UFZ-Verlinkung http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10734
Dautel, F., Kalkhof, S., Trump, S., Michaelson, J., Beyer, A., Lehmann, I., von Bergen, M. (2011):
DIGE-based protein expression analysis of B[a]P-exposed hepatoma cells reveals a complex stress response including alterations in oxidative stress, cell cycle control, and cytoskeleton motility at toxic and subacute concentrations
J. Proteome Res. 10 (2), 379 - 393