Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1007/s00248-010-9645-2
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Titel (primär) Intact phospholipid and quinone biomarkers to assess microbial diversity and redox state in microbial mats
Autor Villanueva, L.; del Campo, J.; Guerrero, R.; Geyer, R.
Quelle Microbial Ecology
Erscheinungsjahr 2010
Department UMB
Band/Volume 60
Heft 1
Seite von 226
Seite bis 238
Sprache englisch
Abstract Microbial mats are stratified microbial communities composed by highly inter-related populations and therefore are frequently chosen as model systems to study diversity and ecophysiological strategies. The present study describes an integrated approach to analyze microbial quinones and intact polar lipids (IPLs) in microbial mats within layers as thin as 500 µm by liquid chromatography-tandem mass spectrometry. Quinone profiles revealed important depth-related differences in community composition in two mat systems. The higher abundance of ubiquinones, compared to menaquinones, reflected the clear predominance of microorganisms belonging to aerobic a-, b-, and g-Proteobacteria in Ebro delta estuarine mats. Hypersaline photosynthetic Camargue mats (France) showed a predominance of menaquinone-9 at the top of the mat, which is consistent with an important contribution of facultative aerobic or anaerobic bacteria in its photic zone. Quinone indices also indicated a higher diversity of non-phototrophs and a more anaerobic character in the hypersaline mats. Besides, the dissimilarity index suggested that the samples were greatly influenced by a depth-related redox state gradient. In the analysis of IPLs, there was a predominance of phosphatidylglycerols and sulfoquinovosyldiacylglycerols, the latter being an abundant biomarker of Cyanobacteria. This combined approach based on quinone and IPL analysis has proven to be a useful method to establish differences in the microbial diversity and redox state of highly structure microbial mat systems at a fine-scale level.
dauerhafte UFZ-Verlinkung
Villanueva, L., del Campo, J., Guerrero, R., Geyer, R. (2010):
Intact phospholipid and quinone biomarkers to assess microbial diversity and redox state in microbial mats
Microb. Ecol. 60 (1), 226 - 238 10.1007/s00248-010-9645-2