Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1038/nmeth.1408
Volltext Shareable Link
Titel (primär) High-throughput generation of selected reaction-monitoring assays for proteins and proteomes
Autor Picotti, P.; Rinner, O.; Stallmach, R.; Dautel, F.; Farrah, T.; Domon, B.; Wenschuh, H.; Aebersold, R.
Quelle Nature Methods
Erscheinungsjahr 2010
Department PROTEOM
Band/Volume 7
Seite von 43
Seite bis 46
Sprache englisch
Supplements https://media.nature.com/original/nature-assets/nmeth/journal/v7/n1/extref/nmeth.1408-S1.pdf
Abstract Selected reaction monitoring (SRM) uses sensitive and specific mass spectrometric assays to measure target analytes across multiple samples, but it has not been broadly applied in proteomics owing to the tedious assay development process for each protein. We describe a method based on crude synthetic peptide libraries for the high-throughput development of SRM assays. We illustrate the power of the approach by generating and applying validated SRM assays for all Saccharomyces cerevisiae kinases and phosphatases.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10391
Picotti, P., Rinner, O., Stallmach, R., Dautel, F., Farrah, T., Domon, B., Wenschuh, H., Aebersold, R. (2010):
High-throughput generation of selected reaction-monitoring assays for proteins and proteomes
Nat. Methods 7 , 43 - 46 10.1038/nmeth.1408