Details zur Publikation

Referenztyp Buchkapitel
DOI / URL Link
Titel (primär) Examination of microbial communities on hydrocarbons by means of laser scanning microscopy
Titel (sekundär) Experimental protocols and appendices
Autor Neu, T.R.; Lawrence, J.R.;
Herausgeber Timmis, K.N.; McGenity, T.; van den Meer, J.R.; de Lorenzo, V.;
Journal / Serie Handbook of Hydrocarbon and Lipid Microbiology
Erscheinungsjahr 2010
Department FLOEK;
Band/Volume Vol. 5, Part 3
Sprache englisch;
UFZ Bestand Leipzig, Bibliothek, Hauptlesesaal, 00358415, 09-1344 DK: 579.222.5 Han
Abstract Microbial communities in their fully hydrated state can be imaged in space and time (4-dimensionally) by laser scanning microscopy using 1-photon or 2-photon excitation. In this chapter we provide guidance on how to examine microorganisms associated with the degradation of liquid, viscous and solid hydrocarbons. The details of mounting samples for upright and inverted microscopes as well as options for fluorescence staining of bacteria and hydrocarbons are presented. Then suggestions are made for recording images and subsequent digital image analysis. Finally practical aspects are discussed such as the material and software needed, the time required and a guide for trouble shooting.
ID 10351
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10351
Neu, T.R., Lawrence, J.R. (2010):
Examination of microbial communities on hydrocarbons by means of laser scanning microscopy
In: Timmis, K.N., McGenity, T., van den Meer, J.R., de Lorenzo, V. (eds.)
Experimental protocols and appendices
Handbook of Hydrocarbon and Lipid Microbiology Vol. 5, Part 3
Springer International Publishing, Cham, p. 4074 - 4084