Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Buchkapitel |
| DOI | 10.1007/978-3-540-77587-4_317 |
| Titel (primär) | Examination of microbial communities on hydrocarbons by means of laser scanning microscopy |
| Titel (sekundär) | Experimental protocols and appendices |
| Autor | Neu, T.R.; Lawrence, J.R. |
| Herausgeber | Timmis, K.N.; McGenity, T.; van den Meer, J.R.; de Lorenzo, V. |
| Quelle | Handbook of Hydrocarbon and Lipid Microbiology |
| Erscheinungsjahr | 2010 |
| Department | FLOEK |
| Band/Volume | Vol. 5, Part 3 |
| Seite von | 4074 |
| Seite bis | 4084 |
| Sprache | englisch |
| UFZ Bestand | Leipzig, Bibliothek, Hauptlesesaal, 00358415, 09-1344 DK: 579.222.5 Han |
| Abstract | Microbial communities in their fully hydrated state can be imaged in space and time (4-dimensionally) by laser scanning microscopy using 1-photon or 2-photon excitation. In this chapter we provide guidance on how to examine microorganisms associated with the degradation of liquid, viscous and solid hydrocarbons. The details of mounting samples for upright and inverted microscopes as well as options for fluorescence staining of bacteria and hydrocarbons are presented. Then suggestions are made for recording images and subsequent digital image analysis. Finally practical aspects are discussed such as the material and software needed, the time required and a guide for trouble shooting. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10351 |
| Neu, T.R., Lawrence, J.R. (2010): Examination of microbial communities on hydrocarbons by means of laser scanning microscopy In: Timmis, K.N., McGenity, T., van den Meer, J.R., de Lorenzo, V. (eds.) Experimental protocols and appendices Handbook of Hydrocarbon and Lipid Microbiology Vol. 5, Part 3 Springer International Publishing, Cham, p. 4074 - 4084 10.1007/978-3-540-77587-4_317 |
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