Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Biosensor online control of citric acid production from glucose by Yarrowia lipolytica using semicontinuous fermentation
Autor Moeller, L.; Grünberg, M.; Zehnsdorf, A.; Strehlitz, B.; Bley, T.;
Journal / Serie Engineering in Life Sciences
Erscheinungsjahr 2010
Department UBZ;
Band/Volume 10
Heft 4
Sprache englisch;
Keywords Biosensor online control; Citric acid; Yarrowia lipolytica
Abstract Our study aimed at the development of an effective method for citric acid production from glucose by use of the yeast Yarrowia lipolytica. The new method included an automated bioprocess control using a glucose biosensor. Several fermentation methodologies including batch, fed-batch, repeated batch and repeated fed-batch cultivation were tested. The best results were achieved during repeated fed-batch cultivation: Within 3 days of cycle duration, approximately 100?g/L citric acid were produced. The yields reached values between 0.51 and 0.65?g/g and the selectivity of the bioprocess for citric acid was as high as 94%. Due to the elongation of the production phase of the bioprocess with growth-decoupled citric acid production, and by operating the fermentation in cycles, an increase in citric acid production of 32% was achieved compared with simple batch fermentation.
ID 10312
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10312
Moeller, L., Grünberg, M., Zehnsdorf, A., Strehlitz, B., Bley, T. (2010):
Biosensor online control of citric acid production from glucose by Yarrowia lipolytica using semicontinuous fermentation
Eng. Life Sci. 10 (4), 311 - 320