Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Degradation of the drug sodium diclofenac by Trametes versicolor pellets and identification of some intermediates by NMR
Autor Marco-Urrea, E.; Pérez-Trujillo, M.; Cruz-Morató, C.; Caminal, G.; Vicent, T.;
Journal / Serie Journal of Hazardous Materials
Erscheinungsjahr 2010
Department ISOBIO;
Band/Volume 176
Heft 1-3
Sprache englisch;
Keywords Diclofenac; Degradation; Laccase; Trametes versicolor; White-rot fungus; NMR
Abstract Degradation of diclofenac sodium, a nonsteroidal anti-inflammatory drug widely found in the aquatic environment, was assessed using the white-rot fungus Trametes versicolor. Almost complete diclofenac removal (=94%) occurred the first hour with T. versicolor pellets when the drug was added at relatively high (10 mg L-1) and environmentally relevant low (45 µg L-1) concentrations in a defined liquid medium. In vivo and in vitro experiments using the cytochrome P450 inhibitor 1-aminobenzotriazole and purified laccase, respectively, suggested at least two different mechanisms employed by T. versicolor to initiate diclofenac degradation. Two hydroxylated metabolites, 4'-hydroxydiclofenac and 5-hydroxydiclofenac, were structurally elucidated by nuclear magnetic resonance as degradation intermediates in fungal cultures spiked with diclofenac. Both parent compound and intermediates disappeared after 24 h leading to a decrease in ecotoxicity calculated by the Microtox test. Laccase-catalyzed transformation of diclofenac led to the formation of 4-(2,6-dichlorophenylamino)-1,3-benzenedimethanol, which was not detected in in vivo experiments probably due to the low laccase activity levels observed through the first hours of incubation.
ID 10273
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10273
Marco-Urrea, E., Pérez-Trujillo, M., Cruz-Morató, C., Caminal, G., Vicent, T. (2010):
Degradation of the drug sodium diclofenac by Trametes versicolor pellets and identification of some intermediates by NMR
J. Hazard. Mater. 176 (1-3), 836 - 842